ReviewExperimental and molecular pathology2008
High resolution melting applications for clinical laboratory medicine.
Review in Experimental and molecular pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
101 citing papers in PubMed, 2 syntheses or guidelines pooled it, 279 citations in OpenAlex.
- Diagnostic accuracy of high-resolution melting curve analysis for discrimination of oncology-associatedThe Journal of international medical research · 2025Pooled it
- Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.PloS one · 2011Pooled it
- Performance of High-Resolution Melting Analysis for Molecular Detection and Species Differentiation of Malaria: A Scoping Review.Pathogens (Basel, Switzerland) · 2026Article
- High-resolution melting analysis for rapid and cost-effective detection of unnatural base pairs.Biodesign research · 2026Article
- First report ofOpen veterinary journal · 2026Article
- Article
- Facile solid-liquid biphasic PCR platform for ultrasensitive multiplex detection and genotyping of HPV.Mikrochimica acta · 2025Article
- High-resolution melting (HRM) method for genotyping Kappa-Casein (CSN3) alleles in buffaloes.Molecular biology reports · 2025Article
- High Prevalence of Autosomal Recessive Alport Syndrome in Roma Population of Eastern Slovakia.Biomedicines · 2025Article
- High-Resolution Melting assays development for discrimination of fungal pathogens causing Grapevine Trunk Diseases.PloS one · 2025Article
- Tag, you're it!: viral diseases in native otters of south-central Chile due to coexistence with invasive American mink and domestic dogs.Frontiers in veterinary science · 2025Article
- Molecular methods in cancer diagnostics: a short review.Annals of medicine · 2024Review
- High-resolution melting assay for rapid, simultaneous detection ofJournal of clinical pathology · 2024Article
- Gene polymorphisms of TACR1 serve as the potential pharmacogenetic predictors of response to the neurokinin-1 receptor antagonist-based antiemetic regimens: a candidate-gene association study in breast cancer patients.Cancer chemotherapy and pharmacology · 2024Article
- L-DNA-Based Melt Analysis Enables Within-Sample Validation of PCR Products.Analytical chemistry · 2024Article
- FUT1 variants responsible for Bombay or para-Bombay phenotypes in a database.Scientific reports · 2023Article
- Development of a high-resolution melt-based assay to rapidly detect the azole-resistantCurrent medical mycology · 2023Article
- Article
- Real-Time Temperature Sensing Using a Ratiometric Dual Fluorescent Protein Biosensor.Biosensors · 2023Article
- Body Adiposity Partially Mediates the Association betweenChildren (Basel, Switzerland) · 2023Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA that is conveniently monitored with fluorescence. Conventional melting is performed after PCR on any real-time instrument to monitor product purity (dsDNA dyes) and sequence (hybridization probes). Recent advances include high resolution instruments and saturating DNA dyes that distinguish many different species. For example, mutation scanning (identifying heterozygotes) by melting is closed-tube and has similar or superior sensitivity and specificity compared to methods that require physical separation. With high resolution melting, SNPs can be genotyped without probes and more complex regions can be typed with unlabeled hybridization probes. Highly polymorphic HLA loci can be melted to establish sequence identity for transplantation matching. Simultaneous genotyping with one or more unlabeled probes and mutation scanning of the entire amplicon can be performed at the same time in the same tube, vastly decreasing or eliminating the need for re-sequencing in genetic analysis. High resolution PCR product melting is homogeneous, closed-tube, rapid (1-5 min), non-destructive and does not require covalently-labeled fluorescent probes. In the clinical laboratory, it is an ideal format for in-house testing, with minimal cost and time requirements for new assay development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.