ArticleDNA repair2008
Telomerase-dependent and -independent chromosome healing in mouse embryonic stem cells.
Article in DNA repair, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Centromere scission drives chromosome shuffling and reproductive isolation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Homologous recombination repair intermediates promote efficient de novo telomere addition at DNA double-strand breaks.Nucleic acids research · 2020Article
- Article
- Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair.Journal of cell science · 2018Article
- A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.Molecular and cellular biology · 2018Article
- Telomeres and genomic evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Article
- Human MLH1 suppresses the insertion of telomeric sequences at intra-chromosomal sites in telomerase-expressing cells.Nucleic acids research · 2017Article
- Processing by MRE11 is involved in the sensitivity of subtelomeric regions to DNA double-strand breaks.Nucleic acids research · 2015Article
- Protection of Drosophila chromosome ends through minimal telomere capping.Journal of cell science · 2015Article
- Regulation of telomere addition at DNA double-strand breaks.Chromosoma · 2013Review
- Suppression of chromosome healing and anticheckpoint pathways in yeast postsenescence survivors.Genetics · 2013Article
- The role of ATM in the deficiency in nonhomologous end-joining near telomeres in a human cancer cell line.PLoS genetics · 2013Article
- Telomere length homeostasis and telomere position effect on a linear human artificial chromosome are dictated by the genetic background.Nucleic acids research · 2012Article
- Telomere dysfunction and chromosome instability.Mutation research · 2012Review
- Mechanisms of telomere loss and their consequences for chromosome instability.Frontiers in oncology · 2012Article
- Article
- Parameters affecting telomere-mediated chromosomal truncation in Arabidopsis.The Plant cell · 2011Article
- Subtelomeric regions in mammalian cells are deficient in DNA double-strand break repair.DNA repair · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Telomeres play an important role in protecting the ends of chromosomes and preventing chromosome fusion. We have previously demonstrated that double-strand breaks near telomeres in mammalian cells result in either the addition of a new telomere at the site of the break, termed chromosome healing, or sister chromatid fusion that initiates chromosome instability. In the present study, we have investigated the role of telomerase in chromosome healing and the importance of chromosome healing in preventing chromosome instability. In embryonic stem cell lines that are wild type for the catalytic subunit of telomerase (TERT), chromosome healing at I-SceI-induced double-strand breaks near telomeres accounted for 22 of 35 rearrangements, with the new telomeres added directly at the site of the break in all but one instance. In contrast, in two TERT-knockout embryonic stem cell lines, chromosome healing accounted for only 1 of 62 rearrangements, with a 23 bp insertion at the site of the sole chromosome-healing event. However, in a third TERT-knockout embryonic stem cell line, 10PTKO-A, chromosome healing was a common event that accounted for 20 of 34 rearrangements. Although this chromosome healing also occurred at the I-SceI site, differences in the microhomology at the site of telomere addition demonstrated that the mechanism was distinct from that in wild-type embryonic stem cell lines. In addition, the newly added telomeres in 10PTKO-A shortened with time in culture, eventually resulting in either telomere elongation through a telomerase-independent mechanism or loss of the subtelomeric plasmid sequences entirely. The combined results demonstrate that chromosome healing can occur through both telomerase-dependent and -independent mechanisms, and that although both mechanisms can prevent degradation and sister chromatid fusion, neither mechanism is efficient enough to prevent sister chromatid fusion from occurring in many cells experiencing double-strand breaks near telomeres.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.