ArticleCellular and molecular neurobiology2008
Combining voltage and calcium imaging from neuronal dendrites.
Article in Cellular and molecular neurobiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.Neurophotonics · 2026Article
- Dendritic excitations govern back-propagation via a spike-rate accelerometer.Nature communications · 2025Article
- Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- Fluorescence lifetime imaging reveals regulation of presynaptic CaJournal of neurochemistry · 2021Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Two Distinct Sets of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Imaging Voltage in Genetically Defined Neuronal Subpopulations with a Cre Recombinase-Targeted Hybrid Voltage Sensor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Functional coupling of diverse voltage-gated Ca(2+) channels underlies high fidelity of fast dendritic Ca(2+) signals during burst firing.The Journal of physiology · 2016Article
- Using simultaneous voltage and calcium imaging to study fast Ca(2+) channels.Neurophotonics · 2015Article
- Combining Membrane Potential Imaging with Other Optical Techniques.Advances in experimental medicine and biology · 2015Review
- Shift and Mean Algorithm for Functional Imaging with High Spatio-Temporal Resolution.Frontiers in cellular neuroscience · 2015Article
- Contribution of sublinear and supralinear dendritic integration to neuronal computations.Frontiers in cellular neuroscience · 2015Review
- Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines.Advances in experimental medicine and biology · 2015Review
- Imaging fast calcium currents beyond the limitations of electrode techniques.Biophysical journal · 2014Article
- Fast state-space methods for inferring dendritic synaptic connectivity.Journal of computational neuroscience · 2014Article
- Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Combined optogenetics and voltage sensitive dye imaging at single cell resolution.Frontiers in cellular neuroscience · 2014Article
- Combining calcium imaging with other optical techniques.Cold Spring Harbor protocols · 2013Review
- Combining Ca2+ and membrane potential imaging in single neurons.Cold Spring Harbor protocols · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The ability to monitor membrane potential (V(m)) and calcium (Ca(2+)) transients at multiple locations on the same neuron can facilitate further progress in our understanding of neuronal function. Here we describe a method to combine V(m) and Ca(2+) imaging using styryl voltage sensitive dyes and Fura type UV-excitable Ca(2+) indicators. In all cases V(m) optical signals are linear with membrane potential changes, but the calibration of optical signals on an absolute scale is presently possible only in some neurons. The interpretation of Ca(2+) optical signals depends on the indicator Ca(2+) buffering capacity relative to the cell endogenous buffering capacity. In hippocampal CA1 pyramidal neurons, loaded with JPW-3028 and 300 microM Bis-Fura-2, V(m) optical signals cannot be calibrated and the physiological Ca(2+) dynamics are compromised by the presence of the indicator. Nevertheless, at each individual site, relative changes in V (m) and Ca(2+) fluorescence signals under different conditions can provide meaningful new information on local dendritic integration. In cerebellar Purkinje neurons, loaded with JPW-1114 and 1 mM Fura-FF, V(m) optical signals can be calibrated in terms of mV and Ca(2+) optical signals quantitatively reveal the physiological changes in free Ca(2+). Using these two examples, the method is explained in detail.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.