ArticleNature structural & molecular biology2008
Two distinct mechanisms generate endogenous siRNAs from bidirectional transcription in Drosophila melanogaster.
Article in Nature structural & molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 106 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
106 citing papers in PubMed, 214 citations in OpenAlex.
- Divergent regulatory impacts of endogenous siRNAs on host mRNAs in testis of closely related species.bioRxiv : the preprint server for biology · 2026Article
- MiSiPi.Rna: an integrated tool for characterizing small regulatory RNA processing.RNA (New York, N.Y.) · 2026Article
- Recurrent expansion and rapid evolution of the Drosophilid RNAi pathway in testis.bioRxiv : the preprint server for biology · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Targeting Regulatory Noncoding RNAs in Human Cancer: The State of the Art in Clinical Trials.Pharmaceutics · 2025Review
- Article
- Review
- Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans.PLoS biology · 2023Article
- Transposon control as a checkpoint for tissue regeneration.Development (Cambridge, England) · 2022Article
- Diverse cell-specific patterns of alternative polyadenylation in Drosophila.Nature communications · 2022Article
- Testing the Drosophila maternal haploid gene for functional divergence and a role in hybrid incompatibility.G3 (Bethesda, Md.) · 2022Article
- Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- The Diversity of Viral Community in Invasive Fruit Flies (Bactrocera and Zeugodacus) Revealed by Meta-transcriptomics.Microbial ecology · 2022Article
- Small RNAs in epigenetic inheritance: from mechanisms to trait transmission.FEBS letters · 2021Review
- Ago2 protectsRNA (New York, N.Y.) · 2021Article
- Trafficking of siRNA precursors by the dsRBD protein Blanks in Drosophila.Nucleic acids research · 2020Article
- Article
- tRNA 2'-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila.Nucleic acids research · 2020Article
- AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.Frontiers in bioscience (Landmark edition) · 2020Review
- Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes.Nucleic acids research · 2019Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cis-natural antisense transcripts (cis-NATs) have been speculated to be substrates for endogenous RNA interference (RNAi), but little experimental evidence for such a pathway in animals has been reported. Analysis of massive Drosophila melanogaster small RNA data sets now reveals two mechanisms that yield endogenous small interfering RNAs (siRNAs) via bidirectional transcription. First, >100 cis-NATs with overlapping 3' exons generate 21-nt, and, based on previously published small RNA data [corrected] Dicer-2 (Dcr-2)-dependent, 3'-end modified siRNAs. The processing of cis-NATs by RNA interference (RNAi) seems to be actively restricted, and the selected loci are enriched for nucleic acid-based functions and include Argonaute-2 (AGO2) itself. Second, we report that extended intervals of the thickveins and klarsicht genes generate exceptionally abundant siRNAs from both strands. These siRNA clusters derive from atypical cis-NAT arrangements involving introns and 5' or internal exons, but their biogenesis is similarly Dcr-2- and AGO2-dependent. These newly recognized siRNA pathways broaden the scope of regulatory networks mediated by small RNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.