ArticleNucleic acids research2008
Regulated gene insertion by steroid-induced PhiC31 integrase.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch.Molecular therapy. Nucleic acids · 2022Article
- Time-Restricted PiggyBac DNA Transposition by Transposase Protein Delivery Using Lentivirus-Derived Nanoparticles.Molecular therapy. Nucleic acids · 2018Article
- Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells.Scientific reports · 2014Article
- DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors.Nucleic acids research · 2014Article
- Article
- Efficient disruption of Zebrafish genes using a Gal4-containing gene trap.BMC genomics · 2013Article
- Conditional gene manipulation: Cre-ating a new biological era.Journal of Zhejiang University. Science. B · 2012Review
- The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.PloS one · 2012Article
- Manipulating piggyBac transposon chromosomal integration site selection in human cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Use of phage φC31 integrase as a tool for zebrafish genome manipulation.Methods in cell biology · 2011Article
- Site-directed integration of transgenes: transposons revisited using DNA-binding-domain technologies.Genetica · 2010Article
- Transgene excision in zebrafish using the phiC31 integrase.Genesis (New York, N.Y. : 2000) · 2010Article
- PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts.BMC biotechnology · 2009Article
- In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonviral integration systems are widely used genetic tools in transgenesis and play increasingly important roles in strategies for therapeutic gene transfer. Methods to efficiently regulate the activity of transposases and site-specific recombinases have important implications for their spatiotemporal regulation in live transgenic animals as well as for studies of their applicability as safe vectors for genetic therapy. In this report, strategies for posttranslational induction of a variety of gene-inserting proteins are investigated. An engineered hormone-binding domain, derived from the human progesterone receptor, hPR891, and specifically recognized by the synthetic steroid mifepristone, is fused to the Sleeping Beauty, Frog Prince, piggyBac and Tol2 transposases as well as to the Flp and PhiC31 recombinases. By analyzing mifepristone-directed inducibility of gene insertion in cultured human cells, efficient posttranslational regulation of the Flp recombinase and the PhiC31 integrase is documented. In addition, fusion of the PhiC31 integrase with the ER(T2) modified estrogen receptor hormone-binding domain results in a protein, which is inducible by a factor of 22-fold and retains 75% of the activity of the wild-type protein. These inducible PhiC31 integrase systems are important new tools in transgenesis and in safety studies of the PhiC31 integrase for gene therapy applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.