ArticleVirology journal2008
N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo.
Article in Virology journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Recent Advances in Di-, Tri-Substituted Mono-Thiazoles, and Bis-Thiazoles: Factors Affecting Biological Activities, Future Aspects, and Challenges.Current topics in medicinal chemistry · 2025Review
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- Synthesis of Novel Aminothiazole Derivatives as Promising Antiviral, Antioxidant and Antibacterial Candidates.International journal of molecular sciences · 2022Article
- Discovery of Small Molecules fromLife (Basel, Switzerland) · 2022Article
- Antiviral drug research for Japanese encephalitis: an updated review.Pharmacological reports : PR · 2022Review
- Isatin derivatives as broad-spectrum antiviral agents: the current landscape.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2022Review
- Mechanistic insights into the Japanese encephalitis virus RNA dependent RNA polymerase protein inhibition by bioflavonoids from Azadirachta indica.Scientific reports · 2021Article
- Screening of Natural Extracts for Inhibitors against Japanese Encephalitis Virus Infection.Antimicrobial agents and chemotherapy · 2020Article
- Japanese Encephalitis: A Brief Review on Indian Perspectives.The open virology journal · 2018Review
- Inhibition of Chikungunya Virus Replication by 1-[(2-Methylbenzimidazol-1-yl) Methyl]-2-Oxo-Indolin-3-ylidene] Amino] Thiourea(MBZM-N-IBT).Scientific reports · 2016Article
- Synthesis and antiviral properties of novel indole-based thiosemicarbazides and 4-thiazolidinones.Bioorganic & medicinal chemistry · 2016Article
- Mannich bases in medicinal chemistry and drug design.European journal of medicinal chemistry · 2015Review
- Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques.Iranian journal of pharmaceutical research : IJPR · 2015Article
- Synthesis of thiocarbohydrazide and carbohydrazide derivatives as possible biologically active agents.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2014Article
- Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo.Archives of virology · 2013Article
- Article
- Japanese Encephalitis Vaccines.Journal of bioterrorism & biodefense · 2011Article
- Inhibition of bovine viral diarrhea virus RNA synthesis by thiosemicarbazone derived from 5,6-dimethoxy-1-indanone.Journal of virology · 2011Article
- Inactivation of the tick-borne encephalitis virus by RNA-cleaving compounds.Journal of molecular and genetic medicine : an international journal of biomedical research · 2011Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the early and mid part of 20th century, several reports described the therapeutic effects of N-methylisatin-beta-Thiosemicarbazone (MIBT) against pox viruses, Maloney leukemia viruses and recently against HIV. However, their ability to inhibit flavivirus replication has not been investigated. Hence the present study was designed to evaluate the antiviral activity of 14 MIBT derivatives against Flaviviruses that are prevalent in India such as Japanese Encephalitis Virus (JEV), Dengue-2 (Den-2) and West Nile viruses (WNV).
resultsAmongst the fourteen Mannich bases of MIBT derivatives tested one compound - SCH 16 was able to completely inhibit in vitro Japanese encephalitis virus (JEV) and West Nile virus (WNV) replication. However no antiviral activity of SCH 16 was noted against Den-2 virus replication. This compound was able to inhibit 50% of the plaques (IC50) produced by JEV and WNV at a concentration of 16 microgm/ml (0.000025 microM) and 4 microgm/ml (0.000006 microM) respectively. Furthermore, SCH 16 at a concentration of 500 mg/kg body weight administered by oral route twice daily was able to completely (100%) prevent mortality in mice challenged with 50LD50 JEV by the peripheral route. Our experiments to understand the mechanism of action suggest that SCH 16 inhibited JEV replication at the level of early protein translation.
conclusionOnly one of the 14 isatin derivatives -SCH 16 exhibited antiviral action on JEV and WNV virus infection in vitro. SCH 16 was also found to completely inhibit JEV replication in vivo in a mouse model challenged peripherally with 50LD50 of the virus. These results warrant further research and development on SCH 16 as a possible therapeutic agent.
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