ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2008
Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI-albumin conjugates.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Lung Gene Expression Suggests Roles for Interferon-Stimulated Genes and Adenosine Deaminase Acting against RNA-1 in Pathologic Responses to Diisocyanate.Chemical research in toxicology · 2024Article
- Analysis of Lung Gene Expression Reveals a Role for ClAmerican journal of respiratory cell and molecular biology · 2020Article
- Skin Exposure Contributes to Chemical-Induced Asthma: What is the Evidence? A Systematic Review of Animal Models.Allergy, asthma & immunology research · 2020Review
- Progress in Occupational Asthma.International journal of environmental research and public health · 2020Review
- Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]Analytical biochemistry · 2018Article
- Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ.Journal of immunological methods · 2016Article
- Do Low Molecular Weight Agents Cause More Severe Asthma than High Molecular Weight Agents?PloS one · 2016Article
- Genome-Wide Association Study Identifies Novel Loci Associated With Diisocyanate-Induced Occupational Asthma.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Glutathione reaction products with a chemical allergen, methylene-diphenyl diisocyanate, stimulate alternative macrophage activation and eosinophilic airway inflammation.Chemical research in toxicology · 2015Article
- Connecting glutathione with immune responses to occupational methylene diphenyl diisocyanate exposure.Chemico-biological interactions · 2013Article
- Interferon-γ promoter is hypermethylated in blood DNA from workers with confirmed diisocyanate asthma.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin.Toxicology in vitro : an international journal published in association with BIBRA · 2013Article
- Pathogenesis and disease mechanisms of occupational asthma.Immunology and allergy clinics of North America · 2011Review
- Environmental isocyanate-induced asthma: morphologic and pathogenetic aspects of an increasing occupational disease.International journal of environmental research and public health · 2011Review
- Occupational asthma: etiologies and risk factors.Allergy, asthma & immunology research · 2011Article
- Occupational exposure to HDI: progress and challenges in biomarker analysis.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2010Article
- Pro/Con debate: Is occupational asthma induced by isocyanates an immunoglobulin E-mediated disease?Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2010Review
- Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate.Analytical biochemistry · 2010Article
- Transient receptor potential ankyrin 1 antagonists block the noxious effects of toxic industrial isocyanates and tear gases.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIsocyanates, a leading cause of occupational asthma, are known to induce adaptive immune responses; however, innate immune responses, which generally precede and regulate adaptive immunity, remain largely uncharacterized.
objectiveThe aim of the study was to identify and characterize the cellular, molecular and systemic innate immune responses induced by hexamethylene diisocyanate (HDI).
methodsHuman peripheral blood mononuclear cells (PBMCs) were stimulated in vitro with HDI-albumin conjugates or control antigen, and changes in phenotype, gene and protein expression were characterized by flow cytometry, microarray, Western blot and ELISA. Cell uptake of isocyanate was visualized microscopically using HDI-albumin conjugates prepared with fluorescently labelled albumin. In vivo, human HDI exposure was performed via a specific inhalation challenge, and subsequent changes in PBMCs and serum proteins were measured by flow cytometry and ELISA. Genotypes were determined by PCR.
resultsHuman monocytes take up HDI-albumin conjugates and undergo marked changes in morphology and gene/protein expression in vitro. The most significant (P-values 0.007-0.05) changes in microarray gene expression were noted in lysosomal genes, especially peptidases and proton pumps involved in antigen processing. Chemokines that regulate monocyte/macrophage trafficking (MIF, MCP-1) and pattern-recognition receptors that bind chitin (chitinases) and oxidized low-density lipoprotein (CD68) were also increased following isocyanate-albumin exposure. In vivo, HDI-exposed subjects exhibited a drastic increase in the percentage of PBMCs with the same HDI-albumin responsive phenotype characterized in vitro (HLA-DR(+)/CD11c(+) with altered light scatter properties). An exposure-dependent decrease (46+/-11%; P<0.015) in serum concentrations of chitinase 3-like-1 was also observed in individuals who lack the major (type 1) human chitinase (due to genetic polymorphism), but not in individuals possessing at least one functional chitinase-1 allele.
conclusionsPreviously unrecognized innate immune responses to HDI and HDI-albumin conjugates could influence the clinical spectrum of exposure reactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.