ArticleTraffic (Copenhagen, Denmark)2008
A functional GFP fusion for imaging clathrin-mediated endocytosis.
Article in Traffic (Copenhagen, Denmark), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1.The Journal of cell biology · 2023Article
- Cystin is required for maintaining fibrocystin (FPC) levels and safeguarding proteome integrity in mouse renal epithelial cells: A mechanistic connection between the kidney defects in cpk mice and human ARPKD.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Enhanced incorporation of subnanometer tags into cellular proteins for fluorescence nanoscopy via optimized genetic code expansion.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- An internally eGFP-tagged α-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics.Traffic (Copenhagen, Denmark) · 2020Article
- Nanobodies: Chemical Functionalization Strategies and Intracellular Applications.Angewandte Chemie (International ed. in English) · 2018Review
- Pejvakin, a Candidate Stereociliary Rootlet Protein, Regulates Hair Cell Function in a Cell-Autonomous Manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Green fluorescent protein-tagged apolipoprotein E: A useful marker for the study of hepatic lipoprotein egress.Traffic (Copenhagen, Denmark) · 2017Article
- Single Event Resolution of Plant Plasma Membrane Protein Endocytosis by TIRF Microscopy.Frontiers in plant science · 2017Article
- Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia.PLoS pathogens · 2016Article
- Regulation of fibroblast growth factor receptor signalling and trafficking by Src and Eps8.Journal of cell science · 2013Article
- Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization.International journal of nanomedicine · 2012Article
- Visualizing HIV-1 assembly.Journal of molecular biology · 2011Review
- The AP-2 adaptor beta2 appendage scaffolds alternate cargo endocytosis.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The ability to localize proteins of interest in live cells through imaging inherently fluorescent protein tags has provided an unprecedented level of information on cellular organization. However, there are numerous cases where fluorescent tags alter the localization and/or function of the proteins to which they are appended. Clathrin-mediated endocytosis from the plasma membrane is a physiologically important process evolutionarily conserved from yeast to humans. Some proteins that are associated with the machinery of clathrin-mediated endocytosis have been tagged with fluorescent proteins. However, it has not yet been possible to study this process through a protein marker that is specific to this step and still fully functional when linked to a fluorescent protein. In this study, we present the first demonstration that one of these proteins, in this case a green fluorescent protein (GFP) fusion to alpha-adaptin, a marker of the adaptor protein-2 complex, functionally complements knockdown of endogenous protein through small interfering RNA silencing. GFP-alpha-adaptin, as well as the techniques used to test the fusion protein, represents an important contribution to the cell biologist's toolbox, which will permit a greater understanding of vesicle trafficking in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.