ArticleAnalytical chemistry2008
Fluorescence recovery assay for the detection of protein-DNA binding.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence.Chemical reviews · 2015Review
- Photoaffinity labeling of transcription factors by DNA-templated crosslinking.Chemical science · 2015Article
- A localized surface plasmon resonance imaging instrument for multiplexed biosensing.Analytical chemistry · 2013Article
- Luminescent detection of DNA-binding proteins.Nucleic acids research · 2012Review
- A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We report a novel and straightforward fluorescence recovery assay which enables the detection of protein-DNA interactions and simultaneously determines relative binding affinities of sequence-specific DNA-binding proteins for a variety of DNA sequences in a multiplexed format. The detection of protein-DNA binding is accomplished by monitoring fluorescence recovery during exonuclease digestion of DNA sequences that are modified with fluorophore-quencher pairs. Retardation of fluorescence recovery occurs with binding of the protein to the putative DNA binding element, which arrests exonuclease digestion. The assay detects protein-DNA binding in a homogeneous solution simply, quickly, and reliably without using radioisotopes. Multiplexing is possible by labeling different DNA sequences with spectrally distinct dyes, allowing simultaneous analysis of experimental and control binding reactions in the same mixture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.