ArticleArteriosclerosis, thrombosis, and vascular biology2008
Counter regulatory effects of PKCbetaII and PKCdelta on coronary endothelial permeability.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Protein kinase C and endothelial dysfunction in select vascular diseases.Frontiers in cardiovascular medicine · 2025Review
- Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- PKCδ regulates the vascular biology in diabetic atherosclerosis.Cell communication and signaling : CCS · 2023Review
- Endothelial Protrusions in Junctional Integrity and Barrier Function.Current topics in membranes · 2018Article
- Ischemia/Reperfusion-Induced Translocation of PKCβII to Mitochondria as an Important Mediator of a Protective Signaling Mechanism in an Ischemia-Resistant Region of the Hippocampus.Neurochemical research · 2017Article
- Regulation of pulmonary endothelial barrier function by kinases.American journal of physiology. Lung cellular and molecular physiology · 2016Review
- Subcellular proteomic approach for identifying the signaling effectors of protein kinase C-β₂ under high glucose conditions in human umbilical vein endothelial cells.Molecular medicine reports · 2015Article
- Involvement of Protein Kinase C-δ in Vascular Permeability in Acute Lung Injury.Immune network · 2015Article
- Myosin light chain kinase signaling in endothelial barrier dysfunction.Medicinal research reviews · 2013Review
- Genetic disruption of protein kinase Cδ reduces endotoxin-induced lung injury.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Interplay between FAK, PKCδ, and p190RhoGAP in the regulation of endothelial barrier function.Microvascular research · 2012Review
- Article
- Hyperglycemia and endothelial dysfunction in atherosclerosis: lessons from type 1 diabetes.International journal of vascular medicine · 2012Article
- Leukocyte rolling and adhesion both contribute to regulation of microvascular permeability to albumin via ligation of ICAM-1.American journal of physiology. Cell physiology · 2011Article
- Differential expression of protein kinase C isoforms in coronary arteries of diabetic mice lacking the G-protein Gα11.Cardiovascular diabetology · 2010Article
- The NO cascade, eNOS location, and microvascular permeability.Cardiovascular research · 2010Review
- Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.American journal of physiology. Heart and circulatory physiology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThe aim of this study was to examine the endothelial distribution and activity of selected PKC isoforms in coronary vessels with respect to their functional impact on endothelial permeability under the experimental conditions relevant to diabetes. METHODS AND
resultsEn face immunohistochemistry demonstrated a significant increase of PKC(betaII) and decrease of PKCdelta expression in coronary arterial endothelium of Zucker diabetic rats. To test whether changes in PKC expression alter endothelial barrier properties, we measured the transcellular electric resistance in human coronary microvascular endothelial monolayers and found that either PKC(betaII) overexpression or PKCdelta inhibition disrupted the cell-cell adhesive barrier. Three-dimensional fluorescence microscopy revealed that hyperpermeability was caused by altered PKC activity in association with distinct translocation of PKC(betaII) to the cell-cell junction and PKCdelta localization to the cytosol. Further analyses in fractionated endothelial lysates confirmed the differential redistribution of these isozymes. Additionally, FRET analysis of PKC subcellular dynamics demonstrated a high PKC(betaII) activity at the cell surface and junction, whereas PKCdelta activity is concentrated in intracellular membrane organelles.
conclusionsTaken together, these data suggest that PKC(betaII) and PKCdelta counter-regulate coronary endothelial barrier properties by targeting distinctive subcellular sites. Imbalanced PKC(betaII)/PKCdelta expression and activity may contribute to endothelial hyperpermeability and coronary dysfunction in diabetes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.