ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Observation of calcium microdomains at the uropod of living morphologically polarized human neutrophils using flash lamp-based fluorescence microscopy.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Downregulation of type 3 inositol (1,4,5)-trisphosphate receptor decreases breast cancer cell migration through an oscillatory CaOncotarget · 2017Article
- Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy.Scientific reports · 2016Article
- Protocol for Biomarker Ratio Imaging Microscopy with Specific Application to Ductal CarcinomaFrontiers in cell and developmental biology · 2016Article
- Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte migration and polarity.Molecular and cellular biology · 2014Article
- Chemokines, selectins and intracellular calcium flux: temporal and spatial cues for leukocyte arrest.Frontiers in immunology · 2012Article
- Migrating human neutrophils exhibit dynamic spatiotemporal variation in membrane lipid organization.American journal of respiratory cell and molecular biology · 2010Article
- Improved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophils.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Calicum microdomains form within neutrophils at the neutrophil-tumor cell synapse: role in antibody-dependent target cell apoptosis.Cancer immunology, immunotherapy : CII · 2010Article
- Neutrophil microdomains: linking heterocellular interactions with vascular injury.Current opinion in hematology · 2010Review
- A cell permeant peptide containing the cytoplasmic tail sequence of Fc receptor type IIA reduces calcium signaling and phagolysosome formation in neutrophils.Cellular immunology · 2010Article
- The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum.Cell motility and the cytoskeleton · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The present study outlines improved strategies for ratiometric imaging of cell calcium using a flash lamp-based excitation method and its application to neutrophil polarization. A brief (approximately 6 micros) and intense flash was used to excite the Fluo-4 and Fura Red calcium dye combination in morphologically polarized human neutrophils. These illumination conditions do not allow the dye or calcium ions to diffuse significant distances during the exposure period. Buffer conditions such as pH, pyruvate concentration, and glucose levels were adjusted to more faithfully replicate these parameters in sepsis patients. Fluorescence images at both dyes' emission wavelengths were simultaneously collected using a Dual-View apparatus and an ICCD camera. The ratiometric images, when viewed as single frames or averaged image stacks, clearly demonstrated high calcium probe ratios at the uropod and comparatively low ratios at the cell body that were not evident using conventional imaging methods with longer exposure times. Calcium signaling at the uropod is likely associated with cytoskeletal remodeling during cell motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.