ArticleProceedings of the National Academy of Sciences of the United States of America1991
Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 358 citations in OpenAlex.
- ETAmerican journal of physiology. Heart and circulatory physiology · 2021Trial
- Endothelin and myocardial ischemia.Cardiovascular drugs and therapy · 1994Trial
- Antecubital venous endothelial ETB receptor protein expression is preserved with aging in men.American journal of physiology. Heart and circulatory physiology · 2024Article
- ETAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Observational
- Article
- Interplay between endothelin and erythropoietin in astroglia: the role in protection against hypoxia.International journal of molecular sciences · 2014Article
- Cloning expeditions: risky but rewarding.Molecular and cellular biology · 2013Article
- Androgens influence microvascular dilation in PCOS through ET-A and ET-B receptors.American journal of physiology. Endocrinology and metabolism · 2013Article
- Proximal nephron.Comprehensive Physiology · 2013Review
- Endothelin B receptor contribution to peripheral microvascular function in women with polycystic ovary syndrome.The Journal of physiology · 2011Article
- Cardiac myofibroblasts isolated from the site of myocardial infarction express endothelin de novo.American journal of physiology. Heart and circulatory physiology · 2003Article
- Developmental changes in the functional, biochemical and molecular properties of rat bladder endothelin receptors.Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- Endothelin antagonists and heart failure.Current hypertension reports · 2002Review
- Electrophysiological analysis of the negative chronotropic effect of endothelin-1 in rabbit sinoatrial node cells.The Journal of physiology · 2001Article
- Endothelin A receptor antagonists in congestive heart failure: blocking the beast while leaving the beauty untouched?Heart failure reviews · 2001Review
- Endotoxin causes up-regulation of endothelin receptors in cultured hepatic stellate cells via nitric oxide-dependent and -independent mechanisms.British journal of pharmacology · 2000Article
- Expression of endothelin receptor subtypes and their messenger RNAs in diabetic rat prostate: effect of insulin treatment.Molecular and cellular biochemistry · 2000Article
- Effects of several endothelin receptor antagonists on gastrointestinal transit of guinea pigs.Digestive diseases and sciences · 2000Article
- Endothelin-1 and endothelin-3 stimulate insulin release by isolated rat pancreatic islets.Journal of endocrinological investigation · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
By screening a cDNA library derived from the A10 rat vascular smooth muscle cell line for functional expression in COS cells, we have isolated a high-affinity receptor for endothelin 1 (Kd = 476 pM) and endothelin 2. The affinity of the cloned endothelin receptor for endothelin 3 is greater than 100 times less in A10 cells and in a CHO cell line stably transformed by the endothelin receptor cDNA. The 426-amino acid receptor polypeptide has seven putative hydrophobic transmembrane domains and is presumed to be a member of the family of guanine nucleotide-binding regulatory (G) protein-coupled receptors. Microinjection of in vitro transcripts of the cloned cDNA into CHO cells confers a transient increase in intracellular calcium in response to endothelin 1, indicating that the receptor is functional and couples to the appropriate G protein(s). RNA analysis reveals high expression in rat lung and heart, tissues known to exhibit binding to iodinated endothelin 1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.