ArticleClinical and vaccine immunology : CVI2008
Double-antigen enzyme-linked immunosorbent assay for detection of hepatitis E virus-specific antibodies in human or swine sera.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera - A phase II study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Hepatitis E Virus Exposure Across Multiple Host Species in a Shared Ecosystem in Argentina.Veterinary sciences · 2026Article
- Absence of anti-HEV antibodies in donkeys in Algeria: a first serological survey.Veterinary research communications · 2026Article
- Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan.Pathogens (Basel, Switzerland) · 2025Article
- Advancing syphilis diagnosis: multi-phase study evaluation of a TpN17-based double-antigen sandwich ELISA for detectingFrontiers in microbiology · 2025Article
- Serological and Molecular Survey of Hepatitis E Virus in Small Ruminants from Central Portugal.Food and environmental virology · 2024Article
- Hepatitis E virus in wild and domestic rabbits from Portugal: a combined molecular and longitudinal serological study.Veterinary research communications · 2024Article
- Epidemiological survey and risk factors associated withOne health (Amsterdam, Netherlands) · 2023Article
- A multispecies competitive nanobody-based ELISA for the detection of antibodies against hepatitis E virus.Scientific reports · 2023Article
- Laboratory Diagnosis of HEV Infection.Advances in experimental medicine and biology · 2023Review
- Monitoring of Hepatitis E Virus in Wild Lagomorphs in Spanish Mediterranean Ecosystems.Transboundary and emerging diseases · 2023Article
- First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria.Frontiers in veterinary science · 2023Article
- Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus.PLoS neglected tropical diseases · 2022Article
- Monitoring of hepatitis E virus in zoo animals from Spain, 2007-2021.Transboundary and emerging diseases · 2022Article
- Serological, Virological Investigation and Hepatic Injury Evaluation for Hepatitis E Virus in Hunting Dogs.Pathogens (Basel, Switzerland) · 2022Article
- Hepatitis E virus in the endangered Iberian lynx (Lynx pardinus).Transboundary and emerging diseases · 2022Article
- Epidemiological survey and risk factors associated with hepatitis E virus in small ruminants in southern Spain.Zoonoses and public health · 2022Article
- Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera.PLoS neglected tropical diseases · 2022Article
- Sero-prevalence of Newcastle disease and associated risk factors in chickens at backyard chicken production Kindo Koisha, Wolaita zone, Southern Ethiopia.Frontiers in veterinary science · 2022Article
- Advances in Hepatitis E Virus Biology and Pathogenesis.Viruses · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new double-antigen sandwich-based enzyme-linked immunosorbent assay (ELISA) for the detection of total antibodies (immunoglobulin G [IgG] and IgM) specific for hepatitis E virus (HEV) was developed by utilizing well-characterized recombinant protein ET2.1 and its peroxidase-labeled counterpart. Our study showed that the ELISA detected all the positive patient samples (n = 265) regardless of whether they contained IgM or IgG antibodies, or both, while it maintained an excellent specificity of 98.8% with samples from various patient or healthy control groups (total number of samples, 424). The test had a detection limit for anti-HEV IgG antibodies that was equivalent to 62 mIU/ml of the international reference. Compared with the serological status of the specimens determined on the basis of tests performed at the individual collection sites, the testing outcome generated by the new ELISA had a good agreement of 99.3%, with a kappa value of 0.985. The positive predictive value and the negative predictive value for the new test reached 98.1% and 100%, respectively. This ELISA had a positive delta value of 4.836 and a negative delta value of 3.314 (where delta is a measure of the number of standard deviations by which the cutoff is separated from the mean of the sample groups) (N. Crofts, W. Maskill, and I. D. Gust, J. Virol. Methods 22:51-59, 1988), indicating that it had an excellent ability to differentiate the infected and noninfected cohorts. Furthermore, the new design enables the detection of antibodies not only in human samples but also in pig samples. Our preliminary data showed that the ELISA could detect seroconversion in samples from pigs at as early as 14 days postinoculation. The potential utility of detecting specific antibodies in pigs will be an added advantage for managing the disease, with suggested zoonotic implications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.