ArticleAmerican journal of physiology. Cell physiology2008
Extracellular matrix-specific focal adhesions in vascular smooth muscle produce mechanically active adhesion sites.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 127 citations in OpenAlex.
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- Graph Neural Networks Reveal Candidate Protein Biomarkers Underlying Abdominal Aortic Aneurysm Biology.Computational and structural biotechnology journal · 2026Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Study of the Effect of Cell Prestress on the Cell Membrane Penetration Behavior by Atomic Force Microscopy.Micromachines · 2023Article
- Myosin light chain phosphorylation exhibits a gradient across the wall of cerebellar arteries under sustained ex vivo vascular tone.Scientific reports · 2023Article
- Postnatal development of extracellular matrix and vascular function in small arteries of the rat.Frontiers in pharmacology · 2023Article
- Role of the vascular endothelial sodium channel activation in the genesis of pathologically increased cardiovascular stiffness.Cardiovascular research · 2022Review
- Modification of Fibronectin by Non-Enzymatic Glycation Impairs KFrontiers in physiology · 2022Article
- Using Polyacrylamide Hydrogels to Model Physiological Aortic Stiffness Reveals that Microtubules Are Critical Regulators of Isolated Smooth Muscle Cell Morphology and Contractility.Frontiers in pharmacology · 2022Article
- Integrin α2β1 regulates collagen I tethering to modulate hyperresponsiveness in reactive airway disease models.The Journal of clinical investigation · 2021Article
- Cerebral Autoregulation in Subarachnoid Hemorrhage.Frontiers in neurology · 2021Review
- Biomechanical signal communication in vascular smooth muscle cells.Journal of cell communication and signaling · 2020Review
- Switching behaviour in vascular smooth muscle cell-matrix adhesion during oscillatory loading.Journal of theoretical biology · 2020Article
- The integrin facilitated internalization of fibronectin-functionalized camptothecin-loaded DNA-nanofibers for high-efficiency anticancer effects.Drug delivery and translational research · 2020Article
- Regulation of blood flow in small arteries: mechanosensory events underlying myogenic vasoconstriction.Journal of exercise rehabilitation · 2020Review
- The interplay of membrane cholesterol and substrate on vascular smooth muscle biomechanics.Current topics in membranes · 2020Article
- Extracellular matrix stiffness regulates human airway smooth muscle contraction by altering the cell-cell coupling.Scientific reports · 2019Article
- Membrane cholesterol and substrate stiffness co-ordinate to induce the remodelling of the cytoskeleton and the alteration in the biomechanics of vascular smooth muscle cells.Cardiovascular research · 2019Article
- The interdependent effects of cholesterol and substrate stiffness on vascular smooth muscle cell biomechanics.Cardiovascular research · 2019Article
4 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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Abstract
Integrin-mediated mechanotransduction in vascular smooth muscle cells (VSMCs) plays an important role in the physiological control of tissue blood flow and vascular resistance. To test whether force applied to specific extracellular matrix (ECM)-integrin interactions could induce myogenic-like mechanical activity at focal adhesion sites, we used atomic force microscopy (AFM) to apply controlled forces to specific ECM adhesion sites on arteriolar VSMCs. The tip of AFM probes were fused with a borosilicate bead (2 ~ 5 microm) coated with fibronectin (FN), collagen type I (CNI), laminin (LN), or vitronectin (VN). ECM-coated beads induced clustering of alpha(5)- and beta(3)-integrins and actin filaments at sites of bead-cell contact indicative of focal adhesion formation. Step increases of an upward (z-axis) pulling force (800 ~ 1,600 pN) applied to the bead-cell contact site for FN-specific focal adhesions induced a myogenic-like, force-generating response from the VSMC, resulting in a counteracting downward pull by the cell. This micromechanical event was blocked by cytochalasin D but was enhanced by jasplakinolide. Function-blocking antibodies to alpha(5)beta(1)- and alpha(v)beta(3)-integrins also blocked the micromechanical cell event in a concentration-dependent manner. Similar pulling experiments with CNI, VN, or LN failed to induce myogenic-like micromechanical events. Collectively, these results demonstrate that mechanical force applied to integrin-FN adhesion sites induces an actin-dependent, myogenic-like, micromechanical event. Focal adhesions formed by different ECM proteins exhibit different mechanical characteristics, and FN appears of particular relevance in its ability to strongly attach to VSMCs and to induce myogenic-like, force-generating reactions from sites of focal adhesion in response to externally applied forces.
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