ArticleAmerican journal of physiology. Renal physiology2008
Effects of amiloride, benzamil, and alterations in extracellular Na+ on the rat afferent arteriole and its myogenic response.
Article in American journal of physiology. Renal physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- The Effect of Renal Ischemia/Reperfusion on the Renal Expression of Epithelial Sodium Channels in Rat: Possible Role of Neural Precursor Cell-Expressed Developmentally Down-Regulated Protein (Nedd4-2).Kidney & blood pressure research · 2026Article
- Inhibition of Voltage-Gated NaInternational journal of molecular sciences · 2023Article
- Post-Ganglionic Sympathetic Neurons can Directly Sense Raised Extracellular NaFrontiers in physiology · 2022Article
- Purinoceptors, renal microvascular function and hypertension.Physiological research · 2020Review
- Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells.International journal of molecular sciences · 2020Article
- Tumor necrosis factor-α impairs cerebral blood flow in pregnant rats: role of vascular β-epithelial NaAmerican journal of physiology. Heart and circulatory physiology · 2020Article
- Involvement of Epithelial NaScientific reports · 2017Article
- Amiloride Analogs as ASIC1a Inhibitors.CNS neuroscience & therapeutics · 2016Article
- βENaC acts as a mechanosensor in renal vascular smooth muscle cells that contributes to renal myogenic blood flow regulation, protection from renal injury and hypertension.Journal of nephrology research · 2015Article
- Renal autoregulation in health and disease.Physiological reviews · 2015Review
- Calcium dynamics underlying the myogenic response of the renal afferent arteriole.American journal of physiology. Renal physiology · 2014Article
- P2 receptors in renal autoregulation.Current vascular pharmacology · 2014Review
- Afferent Arteriolar Responses to β,γ-methylene ATP and 20-HETE are not Blocked by ENaC Inhibition.Physiological reports · 2013Article
- Mural propagation of descending vasa recta responses to mechanical stimulation.American journal of physiology. Renal physiology · 2013Article
- Impaired myogenic constriction of the renal afferent arteriole in a mouse model of reduced βENaC expression.American journal of physiology. Renal physiology · 2012Article
- How NaCl raises blood pressure: a new paradigm for the pathogenesis of salt-dependent hypertension.American journal of physiology. Heart and circulatory physiology · 2012Review
- βENaC is a molecular component of a VSMC mechanotransducer that contributes to renal blood flow regulation, protection from renal injury, and hypertension.Frontiers in physiology · 2012Article
- A mathematical model of the myogenic response to systolic pressure in the afferent arteriole.American journal of physiology. Renal physiology · 2011Article
- Altered whole kidney blood flow autoregulation in a mouse model of reduced beta-ENaC.American journal of physiology. Renal physiology · 2010Article
- Complex interactions of NO/cGMP/PKG systems on Ca2+ signaling in afferent arteriolar vascular smooth muscle.American journal of physiology. Heart and circulatory physiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have implicated epithelial Na+ channels (ENaC) in myogenic signaling. The present study was undertaken to determine if ENaC and/or Na+ entry are involved in the myogenic response of the rat afferent arteriole. Myogenic responses were assessed in the in vitro hydronephrotic kidney model. ENaC expression and membrane potential responses were evaluated with afferent arterioles isolated from normal rat kidneys. Our findings do not support a role of ENaC, in that ENaC channel blockers did not reduce myogenic responses and ENaC expression could not be demonstrated in this vessel. Reducing extracellular Na+ concentration ([Na+]o; 100 mmol/l) did not attenuate myogenic responses, and amiloride had no effect on membrane potential. Benzamil, an inhibitor of ENaC that also blocks Na+/Ca2+ exchange (NCX), potentiated myogenic vasoconstriction. Benzamil and low [Na+]o elicited vasoconstriction; however, these responses were attenuated by diltiazem and were associated with significant membrane depolarization, suggesting a contribution of mechanisms other than a reduction in NCX. Na+ repletion induced a vasodilation in pressurized afferent arterioles preequilibrated in low [Na+]o, a hallmark of NCX, and this response was reduced by 10 micromol/l benzamil. The dilation was eliminated, however, by a combination of benzamil plus ouabain, suggesting an involvement of the electrogenic Na+-K+-ATPase. In concert, these findings refute the premise that ENaC plays a significant role in the rat afferent arteriole and instead suggest that reducing [Na+](o) and/or Na+ entry is coupled to membrane depolarization. The mechanisms underlying these unexpected and paradoxical effects of Na+ are not resolved at the present time.
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