ArticleProtein expression and purification2008
Bacterial expression and purification of interleukin-2 tyrosine kinase: single step separation of the chaperonin impurity.
Article in Protein expression and purification, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Continuous evolution of a halogenase enzyme with improved solubility and activity for sustainable bioproduction.Nature communications · 2026Article
- Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering.Applied microbiology and biotechnology · 2025Article
- Bacterial Production of CDKL5 Catalytic Domain: Insights in Aggregation, Internal Translation and Phosphorylation Patterns.International journal of molecular sciences · 2024Article
- Saturation mutagenesis of a predicted ancestral Syk-family kinase.Protein science : a publication of the Protein Society · 2022Article
- The Family Keeps on Growing: Four Novel Fungal OYEs Characterized.International journal of molecular sciences · 2022Article
- Optimizing Chaperone Removal Strategy from Overexpressed Recombinant Proteins : GNE, a Case Study.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Consideration on Efficient Recombinant Protein Production: Focus on Substrate Protein-Specific Compatibility Patterns of Molecular Chaperones.The protein journal · 2021Article
- Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction.Molecules (Basel, Switzerland) · 2021Article
- A novel method for removing contaminant Hsp70 molecular chaperones from recombinant proteins.Protein science : a publication of the Protein Society · 2019Article
- An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition.Biochemistry · 2017Article
- A simple and efficient method for generating high-quality recombinant Mical enzyme for in vitro assays.Protein expression and purification · 2016Article
- Development of Poly Unsaturated Fatty Acid Derivatives of Aspirin for Inhibition of Platelet Function.The Journal of pharmacology and experimental therapeutics · 2016Article
- Dynamic Allostery Mediated by a Conserved Tryptophan in the Tec Family Kinases.PLoS computational biology · 2016Article
- Production and Purification of a Novel Anti-TNF-α Single Chain Fragment Variable Antibody.Advanced pharmaceutical bulletin · 2015Article
- Purification of a Multidrug Resistance Transporter for Crystallization Studies.Antibiotics (Basel, Switzerland) · 2015Article
- The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching.Plant cell reports · 2015Article
- Biochemical Characterization of Putative Adenylate Dimethylallyltransferase and Cytokinin Dehydrogenase from Nostoc sp. PCC 7120.PloS one · 2015Article
- Strep-tag II and Twin-Strep based cassettes for protein tagging by homologous recombination and characterization of endogenous macromolecular assemblies in Saccharomyces cerevisiae.Molecular biotechnology · 2014Article
- Thymine DNA glycosylase is a CRL4Cdt2 substrate.The Journal of biological chemistry · 2014Article
- A conserved water-mediated hydrogen bond network defines bosutinib's kinase selectivity.Nature chemical biology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Biochemical and biophysical characterization of kinases requires large quantities of purified protein. Here, we report the bacterial expression and purification of active Itk kinase domain (a Tec family kinase) using ArcticExpress cells that co-express the chaperonin system Cpn60/10 from Oleispira antarctica. We describe a simple one step MgCl2/ATP/KCl incubation procedure to remove the co-purifying chaperonin impurity. Chaperonin co-purification is a common problem encountered during protein purification and the simple incubation step described here completely overcomes this problem. The approach targets the chaperonin system rather than the protein of interest and is therefore widely applicable to other protein targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.