ArticleExperimental hematology2008
Granulocytic nuclear differentiation of lamin B receptor-deficient mouse EPRO cells.
Article in Experimental hematology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Shaping neutrophil morphology and function: the importance of a segmented nucleus.Nature reviews. Immunology · 2026Review
- Envelope-Limited Chromatin Sheets (ELCS) Formation in The Nuclear Envelope of HL-60/S4 Cells.bioRxiv : the preprint server for biology · 2026Article
- Chromatin phase separation and nuclear shape fluctuations are correlated in a polymer model of the nucleus.Nucleus (Austin, Tex.) · 2024Article
- Loss of myeloid-specific lamin A/C drives lung metastasis through Gfi-1 and C/EBPε-mediated granulocytic differentiation.Molecular carcinogenesis · 2020Article
- Nuclear Deformation During Neutrophil Migration at Sites of Inflammation.Frontiers in immunology · 2018Review
- Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes.Molecular biology of the cell · 2017Article
- Cooperative Activity of GABP with PU.1 or C/EBPε Regulates Lamin B Receptor Gene Expression, Implicating Their Roles in Granulocyte Nuclear Maturation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Article
- Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Lamin B receptor recognizes specific modifications of histone H4 in heterochromatin formation.The Journal of biological chemistry · 2012Article
- Lamin B receptor regulates the growth and maturation of myeloid progenitors via its sterol reductase domain: implications for cholesterol biosynthesis in regulating myelopoiesis.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Nuclear mechanics in disease.Annual review of biomedical engineering · 2011Review
- Severe generalized muscular atrophia, nerve optic atrophia, ear problem and disability with Pelger-Huet anomaly.Caspian journal of internal medicine · 2011Article
- Requirement for lamin B receptor and its regulation by importin {beta} and phosphorylation in nuclear envelope assembly during mitotic exit.The Journal of biological chemistry · 2010Article
- Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.Molecular biology of the cell · 2010Article
- Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure.Chromosoma · 2009Review
- The LINC-less granulocyte nucleus.European journal of cell biology · 2009Article
- Mouse neutrophils lacking lamin B-receptor expression exhibit aberrant development and lack critical functional responses.Experimental hematology · 2008Article
- Dosage effect of zero to three functional LBR-genes in vivo and in vitro.Nucleus (Austin, Tex.)Article
- An in vitro model for Pelger-Huët anomaly: stable knockdown of lamin B receptor in HL-60 cells.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveLamin B receptor (LBR) is an integral protein of the inner nuclear membrane. Recent studies have demonstrated that genetic deficiency of LBR during granulopoiesis results in hypolobulation of the mature neutrophil nucleus, as observed in human Pelger-Huët anomaly and mouse ichthyosis (ic). In this study, we utilized differentiated early promyelocytes (EPRO cells) that were derived from the bone marrow of homozygous and heterozygous ichthyosis mice to examine changes to the expression of nuclear envelope proteins and heterochromatin structure that result from deficient LBR expression. MATERIALS AND
methodsWild-type (+/+), heterozygous (+/ic), and homozygous (ic/ic) granulocytic forms of EPRO cells were analyzed for the expression of multiple lamins and inner nuclear envelope proteins by immunostaining and immunoblotting techniques. The heterochromatin architecture was also examined by immunostaining for histone lysine methylation.
resultsWild-type (+/+) and heterozygous (+/ic) granulocytic forms revealed ring-shaped nuclei and contained LBR within the nuclear envelope; ic/ic granulocytes exhibited smaller ovoid nuclei devoid of LBR. The pericentric heterochromatin of undifferentiated and granulocytic ic/ic cells was condensed into larger spots and shifted away from the nuclear envelope, compared to +/+ and +/ic cell forms. Lamin A/C, which is normally not present in mature granulocytes, was significantly elevated in LBR-deficient EPRO cells.
conclusionsOur observations suggest roles for LBR during granulopoiesis, which can involve augmenting nuclear membrane growth, facilitating compartmentalization of heterochromatin, and promoting downregulation of lamin A/C expression.
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