ArticleBMC genomics2008
A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS).
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Population structure and genetic diversity of a germplasm for hybrid breeding in rye (Secale cereale L.) using high-density DArTseq-based silicoDArT and SNP markers.Journal of applied genetics · 2023Article
- 100 Years of Chromosome Research in Rye,Plants (Basel, Switzerland) · 2022Review
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- The expansion of heterochromatin blocks in rye reflects the co-amplification of tandem repeats and adjacent transposable elements.BMC genomics · 2016Article
- Construction and characterization of three wheat bacterial artificial chromosome libraries.International journal of molecular sciences · 2014Article
- Genome-wide characterization of genetic diversity and population structure in Secale.BMC plant biology · 2014Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Sequence composition and gene content of the short arm of rye (Secale cereale) chromosome 1.PloS one · 2012Article
- BAC libraries from wheat chromosome 7D: efficient tool for positional cloning of aphid resistance genes.Journal of biomedicine & biotechnology · 2011Article
- Article
- Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- DArT markers for the rye genome - genetic diversity and mapping.BMC genomics · 2009Article
- Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Development of microsatellite markers specific for the short arm of rye (Secale cereale L.) chromosome 1.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- A first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R.BMC plant biology · 2008Article
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11 authors.
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Abstract
backgroundGenomics of rye (Secale cereale L.) is impeded by its large nuclear genome (1C approximately 7,900 Mbp) with prevalence of DNA repeats (> 90%). An attractive possibility is to dissect the genome to small parts after flow sorting particular chromosomes and chromosome arms. To test this approach, we have chosen 1RS chromosome arm, which represents only 5.6% of the total rye genome. The 1RS arm is an attractive target as it carries many important genes and because it became part of the wheat gene pool as the 1BL.1RS translocation.
resultsWe demonstrate that it is possible to sort 1RS arm from wheat-rye ditelosomic addition line. Using this approach, we isolated over 10 million of 1RS arms using flow sorting and used their DNA to construct a 1RS-specific BAC library, which comprises 103,680 clones with average insert size of 73 kb. The library comprises two sublibraries constructed using HindIII and EcoRI and provides a deep coverage of about 14-fold of the 1RS arm (442 Mbp). We present preliminary results obtained during positional cloning of the stem rust resistance gene SrR, which confirm a potential of the library to speed up isolation of agronomically important genes by map-based cloning.
conclusionWe present a strategy that enables sorting short arms of several chromosomes of rye. Using flow-sorted chromosomes, we have constructed a deep coverage BAC library specific for the short arm of chromosome 1R (1RS). This is the first subgenomic BAC library available for rye and we demonstrate its potential for positional gene cloning. We expect that the library will facilitate development of a physical contig map of 1RS and comparative genomics of the homoeologous chromosome group 1 of wheat, barley and rye.
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