ArticlePloS one2008
Ubiquitin E3 ligase Ring1b/Rnf2 of polycomb repressive complex 1 contributes to stable maintenance of mouse embryonic stem cells.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 114 citations in OpenAlex.
- Genotranscriptomic meta-analysis of the Polycomb gene CBX2 in human cancers: initial evidence of an oncogenic role.British journal of cancer · 2014Pooled it
- E3 Ubiquitin Ligase Ring Finger Protein 2 Alleviates Cerebral Ischemia-Reperfusion Injury by Stabilizing Mesencephalic Astrocyte-Derived Neurotrophic Factor Through Monoubiquitination.CNS neuroscience & therapeutics · 2024Article
- Acquisition of neural fate by combination of BMP blockade and chromatin modification.iScience · 2023Article
- The Spatiotemporal Expression of Ring1B in the Postnatal Mouse Cochlea.The journal of international advanced otology · 2022Article
- The Polycomb group geneFrontiers in neuroscience · 2022Article
- Does the Global Outbreak of COVID-19 or Other Viral Diseases Threaten the Stem Cell Reservoir Inside the Body?Stem cell reviews and reports · 2021Review
- The Role of E3s in Regulating Pluripotency of Embryonic Stem Cells and Induced Pluripotent Stem Cells.International journal of molecular sciences · 2021Review
- Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Misregulation of ELK1, AP1, and E12 Transcription Factor Networks Is Associated with Melanoma Progression.Cancers · 2020Article
- Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription.Genes · 2019Review
- Association between RNF2+P-AKT expression in pretreatment biopsy specimens, and poor survival following radiotherapy in patients with esophageal squamous cell carcinoma.Oncology letters · 2019Article
- The Role of Ubiquitination in Regulating Embryonic Stem Cell Maintenance and Cancer Development.International journal of molecular sciences · 2019Review
- Loss of the Polycomb group protein Rnf2 results in derepression of tbx-transcription factors and defects in embryonic and cardiac development.Scientific reports · 2019Article
- Implications of CpG islands on chromosomal architectures and modes of global gene regulation.Nucleic acids research · 2018Article
- USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components.Nature communications · 2017Article
- Differential control of retrovirus silencing in embryonic cells by proteasomal regulation of the ZFP809 retroviral repressor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Smarca4 ATPase mutations disrupt direct eviction of PRC1 from chromatin.Nature genetics · 2017Article
- Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states.Nature genetics · 2017Article
- The ubiquitin-proteasome system and chromosome 17 in cerebellar granule cells and medulloblastoma subgroups.Cellular and molecular life sciences : CMLS · 2017Review
- Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.Cell cycle (Georgetown, Tex.) · 2016Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycomb repressive complex 1 (PRC1) core member Ring1b/Rnf2, with ubiquitin E3 ligase activity towards histone H2A at lysine 119, is essential for early embryogenesis. To obtain more insight into the role of Ring1b in early development, we studied its function in mouse embryonic stem (ES) cells. METHODOLOGY/PRINCIPAL
findingsWe investigated the effects of Ring1b ablation on transcriptional regulation using Ring1b conditional knockout ES cells and large-scale gene expression analysis. The absence of Ring1b results in aberrant expression of key developmental genes and deregulation of specific differentiation-related pathways, including TGFbeta signaling, cell cycle regulation and cellular communication. Moreover, ES cell markers, including Zfp42/Rex-1 and Sox2, are downregulated. Importantly, retained expression of ES cell regulators Oct4, Nanog and alkaline phosphatase indicates that Ring1b-deficient ES cells retain important ES cell specific characteristics. Comparative analysis of our expression profiling data with previously published global binding studies shows that the genes that are bound by Ring1b in ES cells have bivalent histone marks, i.e. both active H3K4me3 and repressive H3K27me3, or the active H3K4me3 histone mark alone and are associated with CpG-'rich' promoters. However, deletion of Ring1b results in deregulation, mainly derepression, of only a subset of these genes, suggesting that additional silencing mechanisms are involved in repression of the other Ring1b bound genes in ES cells.
conclusionsRing1b is essential to stably maintain an undifferentiated state of mouse ES cells by repressing genes with important roles during differentiation and development. These genes are characterized by high CpG content promoters and bivalent histone marks or the active H3K4me3 histone mark alone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.