ArticleGenetics2008
Regulators of cellular levels of histone acetylation in Saccharomyces cerevisiae.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- The Yeast Gsk-3 Kinase Mck1 Is Necessary for Cell Wall Remodeling in Glucose-Starved and Cell Wall-Stressed Cells.International journal of molecular sciences · 2025Article
- A transcriptional cofactor regulatory network for the C. elegans intestine.G3 (Bethesda, Md.) · 2023Article
- Acetyl-CoA Metabolism and Histone Acetylation in the Regulation of Aging and Lifespan.Antioxidants (Basel, Switzerland) · 2021Review
- Lysine acetylation regulates the activity of nuclear Pif1.The Journal of biological chemistry · 2020Article
- Review
- A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs.The Journal of biological chemistry · 2019Article
- The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription.Scientific reports · 2017Article
- Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA.Nucleic acids research · 2017Article
- Article
- Article
- The Ccr4-Not complex is a key regulator of eukaryotic gene expression.Wiley interdisciplinary reviews. RNA · 2016Review
- Protein Degradation of RNA Polymerase II-Association Factor 1(PAF1) Is Controlled by CNOT4 and 26S Proteasome.PloS one · 2015Article
- High throughput screening identifies modulators of histone deacetylase inhibitors.BMC genomics · 2014Article
- Chromatin: a capacitor of acetate for integrated regulation of gene expression and cell physiology.Current opinion in genetics & development · 2014Review
- Linking yeast Gcn5p catalytic function and gene regulation using a quantitative, graded dominant mutant approach.PloS one · 2012Article
- Histone deacetylase HD2 interacts with ERF1 and is involved in longan fruit senescence.Journal of experimental botany · 2012Article
- Drosophila models of cardiac disease.Progress in molecular biology and translational science · 2011Review
- A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.Cell · 2010Article
- Epigenome manipulation as a pathway to new natural product scaffolds and their congeners.Natural product reports · 2010Review
- Chemical induction of silent biosynthetic pathway transcription in Aspergillus niger.Journal of industrial microbiology & biotechnology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone acetylation levels are regulated through the opposing activities of histone acetyltransferases (HATs) and deacetylases (HDACs). While much is known about gene-specific control of histone acetylation, little is understood about how total or cellular levels of histone acetylation are regulated. To identify regulators of cellular levels of histone acetylation, we developed an immunofluorescence-based approach to screen the single-gene deletion library of Saccharomyces cerevisiae for strains with significant reductions in cellular histone acetylation levels. Of the 4848 mutants screened, we identified 63 strains with considerable cellular hypoacetylation of N-terminal lysines in histones H3 and H4. The cellular hypoacetylation was validated for subsets of the identified strains through secondary screens including mass spectrometric analysis of individual lysines and chromatin immunoprecipitation of specific genomic loci. Among the identified mutants were several members of the Ccr4-Not complex, V-type ATPases, and vacuolar protein-sorting complexes as well as genes with unknown functions. We show that Gcn5, a major HAT in yeast, has diminished histone acetyltransferase activity in particular mutants, providing a plausible explanation for reduction of cellular acetylation levels in vivo. Our findings have revealed unexpected and novel links between histone acetylation, Gcn5 HAT activity, and diverse processes such as transcription, cellular ion homeostasis, and protein transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.