ArticleGenome biology2008
Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae.
Article in Genome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- The Gti1/Pac2 family protein CFG1 controls fungal chlamydospore formation through orchestrating cell wall remodeling, lipid metabolism, and ribosome biogenesis.Applied and environmental microbiology · 2026Article
- Fungus Derived Indole-3-Acetic Acid Controls Developmental Conidial Death in Magnaporthe oryzae.Molecular plant pathology · 2026Article
- Genome-wide analysis of the B3 family in apple (Malus domestica Borkh.) and functional identification of MdREM19 involvement in salt stress.BMC genomics · 2026Article
- Glutamate dehydrogenase 2 is required for virulence by facilitating fungal growth in the host hemocoel.Virulence · 2025Article
- Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae.Nature microbiology · 2023Article
- Phase-specific transcriptional patterns of the oomycete pathogen Phytophthora sojae unravel genes essential for asexual development and pathogenic processes.PLoS pathogens · 2023Article
- Article
- Contribution of the Tyrosinase (MoTyr) to Melanin Synthesis, Conidiogenesis, Appressorium Development, and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2023Article
- The Devastating Rice Blast Airborne PathogenPathogens (Basel, Switzerland) · 2023Review
- Characterization of a unique polysaccharide monooxygenase from the plant pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Transcriptional Divergence Underpinning Sexual Development in the Fungal Class Sordariomycetes.mBio · 2022Article
- Transcriptional Network inJournal of fungi (Basel, Switzerland) · 2022Article
- Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus.Journal of fungi (Basel, Switzerland) · 2022Article
- Genome Re-Sequencing Reveals the Host-Specific Origin of Genetic Variation inFrontiers in genetics · 2022Article
- Maturation of subtilisin-like protease NbSLP1 from microsporidiaFrontiers in cellular and infection microbiology · 2022Article
- Vacuolar proteases and autophagy in phytopathogenic fungi: A review.Frontiers in fungal biology · 2022Review
- Comparative Transcriptomics and Gene Knockout Reveal Virulence Factors ofJournal of fungi (Basel, Switzerland) · 2021Article
- Diversity and Function of Appressoria.Pathogens (Basel, Switzerland) · 2021Review
- Transcriptional Landscapes of Long Non-coding RNAs and Alternative Splicing inFrontiers in plant science · 2021Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRice blast disease is caused by the filamentous Ascomycetous fungus Magnaporthe oryzae and results in significant annual rice yield losses worldwide. Infection by this and many other fungal plant pathogens requires the development of a specialized infection cell called an appressorium. The molecular processes regulating appressorium formation are incompletely understood.
resultsWe analyzed genome-wide gene expression changes during spore germination and appressorium formation on a hydrophobic surface compared to induction by cAMP. During spore germination, 2,154 (approximately 21%) genes showed differential expression, with the majority being up-regulated. During appressorium formation, 357 genes were differentially expressed in response to both stimuli. These genes, which we refer to as appressorium consensus genes, were functionally grouped into Gene Ontology categories. Overall, we found a significant decrease in expression of genes involved in protein synthesis. Conversely, expression of genes associated with protein and amino acid degradation, lipid metabolism, secondary metabolism and cellular transportation exhibited a dramatic increase. We functionally characterized several differentially regulated genes, including a subtilisin protease (SPM1) and a NAD specific glutamate dehydrogenase (Mgd1), by targeted gene disruption. These studies revealed hitherto unknown findings that protein degradation and amino acid metabolism are essential for appressorium formation and subsequent infection.
conclusionWe present the first comprehensive genome-wide transcript profile study and functional analysis of infection structure formation by a fungal plant pathogen. Our data provide novel insight into the underlying molecular mechanisms that will directly benefit efforts to identify fungal pathogenicity factors and aid the development of new disease management strategies.
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