ArticleStem cells and development2009
Inhibition of ERK promotes collagen gel compaction and fibrillogenesis to amplify the osteogenesis of human mesenchymal stem cells in three-dimensional collagen I culture.
Article in Stem cells and development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- hMSCs form Osteocyte-like Cell Networks within Strain-Stiffening Bottlebrush Polymer Hydrogels.bioRxiv : the preprint server for biology · 2026Article
- Bone repair and key signalling pathways for cell-based bone regenerative therapy: A review.Journal of Taibah University Medical Sciences · 2023Review
- Hesperetin alleviated glucocorticoid-induced inhibition of osteogenic differentiation of BMSCs through regulating the ERK signaling pathway.Medical molecular morphology · 2021Article
- The effects of dynamic compressive loading on human mesenchymal stem cell osteogenesis in the stiff layer of a bilayer hydrogel.Journal of tissue engineering and regenerative medicine · 2019Article
- Article
- Dihydroartemisinin Promotes the Osteogenesis of Human Mesenchymal Stem Cells via the ERK and Wnt/BioMed research international · 2019Article
- The role of hesperetin on osteogenesis of human mesenchymal stem cells and its function in bone regeneration.Oncotarget · 2017Article
- Effects of matrix metalloproteinases on the fate of mesenchymal stem cells.Stem cell research & therapy · 2016Review
- Mesenchymal Stem Cell Spheroids Retain Osteogenic Phenotype Through α2β1 Signaling.Stem cells translational medicine · 2016Article
- Differentiation of Wharton's Jelly-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers.Molecular neurobiology · 2016Article
- Mesenchymal stem cell mechanobiology and emerging experimental platforms.Journal of the Royal Society, Interface · 2013Article
- Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold.Neural regeneration research · 2013Article
- Extracellular matrix protein adsorption to phosphate-functionalized gels from serum promotes osteogenic differentiation of human mesenchymal stem cells.Acta biomaterialia · 2013Article
- Influence of select extracellular matrix proteins on mesenchymal stem cell osteogenic commitment in three-dimensional contexts.Acta biomaterialia · 2012Article
- Tissue Engineering of Blood Vessels: Functional Requirements, Progress, and Future Challenges.Cardiovascular engineering and technology · 2011Article
- Catechin stimulates osteogenesis by enhancing PP2A activity in human mesenchymal stem cells.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2011Article
- Recapitulation of the embryonic cardiovascular progenitor cell niche.Biomaterials · 2011Article
- Effect of phosphatidyl inositol 3-kinase, extracellular signal-regulated kinases 1/2, and p38 mitogen-activated protein kinase inhibition on osteogenic differentiation of muscle-derived stem cells.Tissue engineering. Part A · 2010Article
- Quantification of three-dimensional cell-mediated collagen remodeling using graph theory.PloS one · 2010Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tissue morphogenesis remains one of the least understood problems in cell and developmental biology. There is a disconnect between the mechanisms that apply to two-dimensional (2D) cultures and those seen in vivo. Three-dimensional (3D) culture presents a complex stimulus triggering cellular responses that are only partially understood. We compared 2D and 3D cultures of human mesenchymal stem cells in the presence of mitogen-activated protein kinase kinase (MEK) inhibitor, PD98059, to determine the role of extracellular signal-related kinase (ERK) in collagen-induced differentiation. 3D collagen I culture enhanced and accelerated the osteogenic differentiation of human mesenchymal stem cells (hMSC). Contrary to 2D results, the addition of PD98059 induced a significant amplification of osteogenic gene expression and matrix mineralization in 3D cultures. The inhibition of ERK altered cell-mediated compaction, proliferation, and resulted in the development of distinct tissue microstructure. Therefore, we suggest that the ability to reorganize collagen in 3D is an important step in ERK-mediated osteogenic differentiation. This work aims to propose a correlation between osteogenic differentiation and hMSC-directed collagen I remodeling. We present a potential mechanistic link (ERK) through which the three dimensionality of an engineered tissue acts to differentially induce and maintain cellular phenotype during tissue development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.