ArticleMolecular vision2008
Mitochondrial superoxide dismutase activation with 17 beta-estradiol-treated human lens epithelial cells.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Mechanisms of estrogens' dose-dependent neuroprotective and neurodamaging effects in experimental models of cerebral ischemia.International journal of molecular sciences · 2011Pooled it
- Rosmarinic and Sinapic Acids May Increase the Content of Reduced Glutathione in the Lenses of Estrogen-Deficient Rats.Nutrients · 2019Article
- Effects of Female Sex Steroids Administration on Pathophysiologic Mechanisms in Traumatic Brain Injury.Translational stroke research · 2018Review
- Oestradiol levels and superoxide dismutase activity in age-related cataract: a case-control study.BMC ophthalmology · 2016Article
- Effect of static magnetic fields and phloretin on antioxidant defense system of human fibroblasts.Environmental science and pollution research international · 2016Article
- Antioxidant Expression Response to Free Radicals in Active Men and Women Fallowing to a Session Incremental Exercise; Numerical Relationship Between Antioxidants and Free Radicals.Asian journal of sports medicine · 2016Article
- Article
- Protection against brain tissues oxidative damage as a possible mechanism for improving effects of low doses of estradiol on scopolamine-induced learning and memory impairments in ovariectomized rats.Advanced biomedical research · 2016Article
- Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells.International journal of ophthalmology · 2014Article
- Mitochondrial p38β and manganese superoxide dismutase interaction mediated by estrogen in cardiomyocytes.PloS one · 2014Article
- Quantitative analysis of SOD2, ALDH1A1 and MGST1 messenger ribonucleic acid in anterior lens epithelium of patients with pseudoexfoliation syndrome.Molecular vision · 2013Article
- Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells.Chinese journal of integrative medicine · 2012Article
- Oestrogens ameliorate mitochondrial dysfunction in Leber's hereditary optic neuropathy.Brain : a journal of neurology · 2011Article
- Effects of 17β-estradiol on proliferation, cell viability and intracellular redox status in native human lens epithelial cells.Molecular vision · 2011Article
- Mitochondrial mechanisms of estrogen neuroprotection.Biochimica et biophysica acta · 2010Review
- Expression changes in DNA repair enzymes and mitochondrial DNA damage in aging rat lens.Molecular vision · 2010Article
- Effect of estrogen replacement therapy on lens epithelial cell apoptosis in an experimental rat model.International ophthalmology · 2010Article
- Regulation of mitochondrial respiratory chain biogenesis by estrogens/estrogen receptors and physiological, pathological and pharmacological implications.Biochimica et biophysica acta · 2009Review
- Role of protein phosphatases and mitochondria in the neuroprotective effects of estrogens.Frontiers in neuroendocrinology · 2009Review
- Estrogenic control of mitochondrial function and biogenesis.Journal of cellular biochemistry · 2008Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purpose17 beta-estradiol (17beta-E(2)) protects human lens epithelial cells against oxidative stress by preserving mitochondrial function in part via the non-genomic rapid activation of prosurvival signal transduction pathways. The study described herein examined whether 17beta-E(2) also elicits genomic protection by influencing the expression (and activity) of mitochondrial-associated manganese superoxide dismutase (MnSOD) as a possible parallel mechanism by which 17beta-E(2) protects against oxidative stress.
methodsVirally-transformed human lens epithelial cells (HLE-B3) were pre-incubated with 17beta-E(2), and mRNA or protein lysates were collected over a time course ranging from 90 min to 24 h. Positive expression of lens epithelial cell MnSOD mRNA was determined by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), and its levels were monitored by real-time PCR up to 24 h after 17beta-E(2) administration. Western blot analysis was used to examine the pattern of protein expression as influenced by 17beta-E(2) treatment. MnSOD activity as influenced by 17beta-E(2) was determined by measuring enzymatic activity.
resultsA significant rapid increase in the activity of MnSOD was observed with HLE-B3 cells by 90 min post-bolus addition of 17beta-E(2), which returned to control level by 240 min. Neither an increase in MnSOD mRNA nor in protein expression was detected up through 24 h.
conclusionsThese data demonstrate that 17beta-E(2) rapidly and transiently increases the activity of MnSOD but influences neither its mRNA expression nor its protein expression. The results suggest that (estrogen-activated) MnSOD plays an important role against mitochondrial oxidative stress by diminishing reactive oxygen species, thus promoting cell survival.
Indexed as
Identifiers
18490963PMC2386510W2143685835What OpenQuestion holds
Registered trials
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