ArticleArchives of otolaryngology--head & neck surgery2008
Examination of oral cancer biomarkers by tissue microarray analysis.
Article in Archives of otolaryngology--head & neck surgery, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 63 citations in OpenAlex.
- Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy.Biomolecules · 2021Trial
- Expression analysis of SPARC/osteonectin in oral squamous cell carcinoma patients: from saliva to surgical specimen.BioMed research international · 2013Trial
- A Roadmap for the Rational Use of Biomarkers in Oral Disease Screening.Biomolecules · 2024Review
- Oxidative Stress in the Pathogenesis of Oral Cancer.Biomedicines · 2024Review
- Between the Devil and the Deep Blue Sea: Non-Coding RNAs Associated with Transmissible Cancers in Tasmanian Devil, Domestic Dog and Bivalves.Non-coding RNA · 2021Article
- An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma.Scientific reports · 2021Article
- Deregulation of extracellular matrix modeling with molecular prognostic markers revealed by transcriptome sequencing and validations in Oral Tongue squamous cell carcinoma.Scientific reports · 2021Article
- Pseudogenes: A Novel Source of Trans-Acting Antisense RNAs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Oral cancer diagnosis and perspectives in India.Sensors international · 2020Review
- Proteomic Profiling of Paired Interstitial Fluids Reveals Dysregulated Pathways and Salivary NID1 as a Biomarker of Oral Cavity Squamous Cell Carcinoma.Molecular & cellular proteomics : MCP · 2019Article
- Bioinformatics-based discovery of PYGM and TNNC2 as potential biomarkers of head and neck squamous cell carcinoma.Bioscience reports · 2019Article
- Expression and clinical significance of periostin in oral lichen planus.Experimental and therapeutic medicine · 2018Article
- The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells.Oncology letters · 2018Article
- Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer.International journal of molecular sciences · 2017Article
- Article
- Tenascin-C and carcinoma cell invasion in oral and urinary bladder cancer.Cell adhesion & migration · 2015Review
- Cadherin 11, a miR-675 target, induces N-cadherin expression and epithelial-mesenchymal transition in melasma.The Journal of investigative dermatology · 2014Article
- Silencing Met receptor tyrosine kinase signaling decreased oral tumor growth and increased survival of nude mice.Oral oncology · 2014Article
- Beta-catenin and epithelial tumors: a study based on 374 oropharyngeal cancers.BioMed research international · 2014Article
- TGM3, a candidate tumor suppressor gene, contributes to human head and neck cancer.Molecular cancer · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveTo validate the DNA microarray results on a subset of genes that could potentially serve as biomarkers of oral squamous cell carcinoma (OSCC) by examining their expression with an alternate quantitative method and by assessing their protein levels.
designBased on DNA microarray data from our laboratory and data reported in the literature, we identified 6 potential biomarkers of OSCC to investigate further. We used quantitative real-time polymerase chain reaction to examine expression changes of CDH11, MMP3, SPARC, POSTN, TNC, and TGM3 in OSCC and histologically normal control tissues. We further examined validated markers at the protein level by immunohistochemical analysis of OSCC tissue microarray sections.
resultsQuantitative real-time polymerase chain reaction analysis revealed upregulation of CDH11, SPARC, POSTN, and TNC gene expression and decreased TGM3 expression in OSCC tissue compared with control tissue; MMP3 was not found to be differentially expressed. In tissue microarray immunohistochemical analyses, SPARC (secreted protein, acidic, rich in cysteine), periostin, and tenascin C exhibited increased protein expression in tumor tissue compared with control tissue, and their expression was primarily localized within tumor-associated stroma rather than tumor epithelium. Conversely, transglutaminase 3 protein expression was found only within keratinocytes in control tissue and was significantly downregulated in cancer cells.
conclusionsOf 6 potential gene markers of OSCC, initially identified by DNA microarray analyses, differential expression of CDH11, SPARC, POSTN, TNC, and TGM3 were validated by quantitative real-time polymerase chain reaction. Differential expression and localization of proteins encoded by SPARC, POSTN, TNC, and TGM3 were clearly shown by tissue microarray immunohistochemical analysis.
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