ArticleBMC microbiology2008
High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genome characteristics and type IV effector protein repertoire of Coxiella burnetii depend rather on Genomic Groups than on host species.BMC microbiology · 2026Article
- Pathogen screening of Zambian ticks: new insights on the occurrence of tick-borne pathogens in the country.Parasites & vectors · 2026Article
- Early detection ofMicrobiology spectrum · 2026Article
- Occurrence of viral and bacterial pathogens in South American camelids in representative German flocks.Frontiers in veterinary science · 2026Article
- Current Knowledge, Attitudes, and Practices Toward Ticks and Tick-Borne Zoonoses among Mixed Farming and Pastoral Communities in Gairo and Monduli Districts, Tanzania.The American journal of tropical medicine and hygiene · 2025Article
- Detection ofFoods (Basel, Switzerland) · 2025Article
- Prevalence of tick-borne bacterial pathogens in Germany-has the situation changed after a decade?Frontiers in cellular and infection microbiology · 2024Article
- Tick Species Diversity and Molecular Identification of Spotted Fever Group Rickettsiae Collected from Migratory Birds Arriving from Africa.Microorganisms · 2023Article
- High prevalence ofVeterinary world · 2020Article
- Uptake and fecal excretion of Coxiella burnetii by Ixodes ricinus and Dermacentor marginatus ticks.Parasites & vectors · 2020Article
- Development of a Rapid and Sensitive Colorimetric Loop-Mediated Isothermal Amplification Assay: A Novel Technology for the Detection ofFrontiers in cellular and infection microbiology · 2020Article
- The prevalence of Coxiella burnetii in ticks and animals in Slovenia.BMC veterinary research · 2019Article
- Genetic diversity of Coxiella burnetii in domestic ruminants in central Italy.BMC veterinary research · 2018Article
- False detection of Coxiella burnetii-what is the risk?FEMS microbiology letters · 2016Article
- Estimated herd prevalence and sequence types of Coxiella burnetii in bulk tank milk samples from commercial dairies in Indiana.BMC veterinary research · 2015Article
- Detection of Lyme Disease and Q Fever Agents in Wild Rodents in Central Italy.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2015Article
- Coxiella burnetii in central Italy: novel genotypes are circulating in cattle and goats.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- A case of Q fever prosthetic joint infection and description of an assay for detection of Coxiella burnetii.Journal of clinical microbiology · 2013Article
- Coxiella burnetii causing haemophagocytic syndrome: a rare complication of an unusual pathogen.Infection · 2011Article
- Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands.Applied and environmental microbiology · 2011Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCoxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. Due to its high environmental stability and infectivity it is regarded as a category B biological weapon agent. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation in humans can range from mild flu-like illness to acute pneumonia and hepatitis. Endocarditis represents the most common form of chronic Q-fever. In humans serology is the gold standard for diagnosis but is inadequate for early case detection. In order to serve as a diagnostic tool in an eventual biological weapon attack or in local epidemics we developed a real-time 5'nuclease based PCR assay with an internal control system. To facilitate high-throughput an automated extraction procedure was evaluated.
resultsTo determine the minimum number of copies that are detectable at 95% chance probit analysis was used. Limit of detection in blood was 2,881 copies/ml [95%CI, 2,188-4,745 copies/ml] with a manual extraction procedure and 4,235 copies/ml [95%CI, 3,143-7,428 copies/ml] with a fully automated extraction procedure, respectively. To demonstrate clinical application a total of 72 specimens of animal origin were compared with respect to manual and automated extraction. A strong correlation between both methods was observed rendering both methods suitable. Testing of 247 follow up specimens of animal origin from a local Q-fever epidemic rendered real-time PCR more sensitive than conventional PCR.
conclusionA sensitive and thoroughly evaluated real-time PCR was established. Its high-throughput mode may show a useful approach to rapidly screen samples in local outbreaks for other organisms relevant for humans or animals. Compared to a conventional PCR assay sensitivity of real-time PCR was higher after testing samples from a local Q-fever outbreak.
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