ArticleBiochemistry2008
Influence of phalloidin on the formation of actin filament branches by Arp2/3 complex.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A synthetic coolant (WS-23) in disposable electronic cigarettes impairs cytoskeletal function in EpiAirway microtissues exposed at the air liquid interface.Scientific reports · 2023Article
- S-Benproperine, an Active Stereoisomer of Benproperine, Suppresses Cancer Migration and Tumor Metastasis by Targeting ARPC2.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Natural Bioactive Thiazole-Based Peptides from Marine Resources: Structural and Pharmacological Aspects.Marine drugs · 2020Review
- Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Flow Cytometric Determination of Actin Polymerization in Peripheral Blood Leukocytes Effectively Discriminate Patients With Homozygous Mutation in ARPC1B From Asymptomatic Carriers and Normal Controls.Frontiers in immunology · 2019Article
- Modulation of actin dynamics as potential macrophage subtype-targeting anti-tumour strategy.Scientific reports · 2017Article
- Actin filament curvature biases branching direction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The effect of toxins on inorganic phosphate release during actin polymerization.European biophysics journal : EBJ · 2011Article
- Reconstitution and protein composition analysis of endocytic actin patches.Current biology : CB · 2010Article
- Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The cyclic peptide phalloidin binds and stabilizes actin filaments. It is widely used in studies of actin filament assembly, including analysis of branch formation by Arp2/3 complex, but its influence on the branching reaction has not been considered. Here we show that rhodamine-phalloidin binds both Arp2/3 complex and the VCA domain of Arp2/3 complex activator, hWASp, with dissociation equilibrium constants of about 100 nM. Not only does phalloidin promote nucleation of pure actin monomers but it also dramatically stimulates branch formation by actin, Arp2/3 complex, and hWASp-VCA more than 10-fold and inhibits dissociation of branches. Therefore, the appearance of more branches in samples treated with rhodamine-phalloidin arises from multiple influences of the peptide on both the formation and dissociation of branches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.