ArticleJournal of the American Chemical Society2008
Two color RNA intercalating probe for cell imaging applications.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 167 citations in OpenAlex.
- Metal-free 2-isocyanobiaryl-based cyclization reactions: phenanthridine framework synthesis.Molecular diversity · 2024Review
- Mo-Catalyzed One-Pot Synthesis of N-Polyheterocycles from Nitroarenes and Glycols with Recycling of the Waste Reduction Byproduct. Substituent-Tuned Photophysical Properties.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Oxidative Photocyclization of Aromatic Schiff Bases in Synthesis of Phenanthridines and Other Aza-PAHs.International journal of molecular sciences · 2020Article
- PPhRSC advances · 2020Article
- A side-chain engineering strategy for constructing fluorescent dyes with direct and ultrafast self-delivery to living cells.Chemical science · 2019Article
- Article
- Coupled Excited-State Dynamics in N-Substituted 2-Methoxy-9-Acridones.Frontiers in chemistry · 2019Article
- Crystal structure of (Acta crystallographica. Section E, Crystallographic communications · 2017Article
- Radical Cyclopropanol Ring Opening Initiated Tandem Cyclizations for Efficient Synthesis of Phenanthridines and Oxindoles.Synlett : accounts and rapid communications in synthetic organic chemistry · 2017Article
- Metal-Free Photoredox Catalyzed Cyclization of O-(2,4-Dinitrophenyl)oximes to Phenanthridines.Molecules (Basel, Switzerland) · 2016Article
- Phenoxide-Bridged Zinc(II)-Bis(dipicolylamine) Probes for Molecular Imaging of Cell Death.Bioconjugate chemistry · 2016Article
- Expanding the palette of phenanthridinium cations.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Come-back of phenanthridine and phenanthridinium derivatives in the 21st century.Beilstein journal of organic chemistry · 2014Review
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Uptake and localisation of small-molecule fluorescent probes in living cells: a critical appraisal of QSAR models and a case study concerning probes for DNA and RNA.Histochemistry and cell biology · 2013Review
- Synthesis of phenanthridines via palladium-catalyzed picolinamide-directed sequential C-H functionalization.Beilstein journal of organic chemistry · 2013Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
- FLEth RNA intercalating probe is a convenient reporter for small interfering RNAs.Journal of the American Chemical Society · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Here we report on a phenanthridine derivative which has a covalently linked fluorescein molecule in order to increase the light absorption and hence fluorescence signal intensity when bound to duplex RNA. Steady-state fluorescence shows that the energy transfer efficiency from the fluorescein to the phenanthridine fluorophore is approximately 77%, which results in the probe being over 5x brighter than other phenanthridine derivatives when bound to RNA. Due to the relatively long lifetime (approximately 20 ns) of the probe, time-resolved fluorescence is used to increase the signal to background ratio in cell growth medium from 7 (steady-state value) to over 40. Moreover, fluorescence images of cells containing the probe show that the fluorescein signal is readily apparent along with that of the intercalated fluorophore, allowing this probe to be used as a dual color probe which simultaneously reports the probes' location and that of RNA.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.