ArticleParasitology research2008
Detection of Blastocystis from stool samples using real-time PCR.
Article in Parasitology research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
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- An update on the distribution ofHeliyon · 2022Article
- Comparison of DNA Extraction Methods and Real-Time PCR Assays for the Detection ofMicroorganisms · 2020Article
- Development and evaluation of high-resolution melting curve analysis for rapid detection and subtyping of Blastocystis and comparison the results with sequencing.Parasitology research · 2019Article
- A summary of Blastocystis subtypes in North and South America.Parasites & vectors · 2019Review
- Epidemiological Aspects of Blastocystis Colonization in Children in Ilero, Nigeria.The American journal of tropical medicine and hygiene · 2016Article
- Occurrence ofPeerJ · 2016Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis.Therapeutic advances in infectious disease · 2013Review
- Subtype distribution of Blastocystis isolates identified in a Sydney population and pathogenic potential of Blastocystis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp.Journal of clinical microbiology · 2012Article
- Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.Journal of clinical microbiology · 2011Article
- Molecular characterization of Blastocystis species in Oregon identifies multiple subtypes.Parasitology research · 2010Article
- Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness.Parasitology research · 2009Article
- Oh my aching gut: irritable bowel syndrome, Blastocystis, and asymptomatic infection.Parasites & vectors · 2008Article
- New insights on classification, identification, and clinical relevance of Blastocystis spp.Clinical microbiology reviews · 2008Review
- Epidemiological and diagnostic features of blastocystis infection in symptomatic patients in izmir province, Turkey.Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blastocystis by an ova and parasite test as well as a conventional PCR assay were positive for Blastocystis using our real-time LC PCR assay. Diagnosis of Blastocystis infections using this sensitive method, including DNA extraction and real-time PCR, only requires 3 h. The lower limit of detection for Blastocystis in stool using the real-time LC PCR assay was calculated to be 760 cells of Blastocystis per 100 mg of stool, an estimated 760 parasites per reaction. The assay did not cross-react with Ruminococcus hansenii, Anarococcus hydrogenalis, Bifidobacterium adolescentis, Fusobacterium prausnitzii, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, or Lactobacillus acidophilus. Because of the ease of use, sensitivity, specificity, and increase in Blastocystis infections in the USA we believe this assay has the potential to be useful as a clinical diagnosis tool of Blastocystis infection.
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