ArticleJournal of veterinary science2008
The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin.
Article in Journal of veterinary science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- In vitro and in vivo endothelial interactions of Leptospira species are markers of virulence.PLoS neglected tropical diseases · 2026Article
- Navigating infection by pathogenic spirochetes: The host-bacteria interface at the atomic level.Protein science : a publication of the Protein Society · 2025Review
- GroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophages.BMC microbiology · 2021Article
- Leptospiral Immunoglobulin-Like Domain Proteins: Roles in Virulence and Immunity.Frontiers in immunology · 2020Review
- LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis.PLoS neglected tropical diseases · 2017Article
- Immunoprotective properties of recombinant LigA and LigB in a hamster model of acute leptospirosis.PloS one · 2017Article
- Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum.Immunology letters · 2016Article
- Whole genome sequencing revealed host adaptation-focused genomic plasticity of pathogenic Leptospira.Scientific reports · 2016Article
- DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis.Memorias do Instituto Oswaldo Cruz · 2015Article
- Identification of cell-binding adhesins of Leptospira interrogans.PLoS neglected tropical diseases · 2014Article
- Staphylococcus aureus manganese transport protein C (MntC) is an extracellular matrix- and plasminogen-binding protein.PloS one · 2014Article
- Diagnosis of canine leptospirosis by a highly sensitive FRET-PCR targeting the lig genes.PloS one · 2014Article
- Elastin, a novel extracellular matrix protein adhering to mycobacterial antigen 85 complex.The Journal of biological chemistry · 2013Article
- Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis.PLoS neglected tropical diseases · 2013Article
- Article
- Establishment of a leptospirosis model in guinea pigs using an epicutaneous inoculations route.BMC infectious diseases · 2012Article
- Novel mycobacteria antigen 85 complex binding motif on fibronectin.The Journal of biological chemistry · 2012Article
- Whole genome analysis of Leptospira licerasiae provides insight into leptospiral evolution and pathogenicity.PLoS neglected tropical diseases · 2012Article
- Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.BMC microbiology · 2011Article
- Ca-binding and spectral properties of the common region of surface-exposed Lig proteins of leptospira.Communicative & integrative biology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adhesion through microbial surface components that recognize adhesive matrix molecules is an essential step in infection for most pathogenic bacteria. In this study, we report that LigB interacts with fibronectin (Fn) through its variable region. A possible role for LigB in bacterial attachment to host cells during the course of infection is supported by the following observations: (i) binding of the variable region of LigB to Madin-Darby canine kidney (MDCK) cells in a dose-dependent manner reduces the adhesion of Leptospira, (ii) inhibition of leptospiral attachment to Fn by the variable region of LigB, and (iii) decrease in binding of the variable region of LigB to the MDCK cells in the presence of Fn. Furthermore, we found a significant reduction in binding of the variable region of LigB to Fn using small interfering RNA (siRNA). Finally, the isothermal titration calorimetric results confirmed the interaction between the variable region of LigB and Fn. This is the first report to demonstrate that LigB binds to MDCK cells. In addition, the reduction of Fn expression in the MDCK cells, by siRNA, reduced the binding of LigB. Taken together, the data from the present study showed that LigB is a Fn-binding protein of pathogenic Leptospira spp. and may play a pivotal role in Leptospira-host interaction during the initial stage of infection.
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