ArticleNucleic acids research2008
Recruitment of RNA polymerase III in vivo.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- A Combinatorial Regulatory Platform Determines Expression of RNA Polymerase III Subunit RPC7α (Cancers · 2023Article
- A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA.Nature communications · 2022Article
- Pol3Base: a resource for decoding the interactome, expression, evolution, epitranscriptome and disease variations of Pol III-transcribed ncRNAs.Nucleic acids research · 2022Article
- RNA polymerase III transcription and cancer: A tale of two RPC7 subunits.Frontiers in molecular biosciences · 2022Review
- Structure of human RNA polymerase III elongation complex.Cell research · 2021Article
- Structural insights into transcriptional regulation of human RNA polymerase III.Nature structural & molecular biology · 2021Article
- Prepartal Energy Intake Alters Blood Polymorphonuclear Leukocyte Transcriptome During the Peripartal Period in Holstein Cows.Bioinformatics and biology insights · 2017Article
- Telomerase reverse transcriptase promotes cancer cell proliferation by augmenting tRNA expression.The Journal of clinical investigation · 2016Article
- Characterization of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome.Scientific reports · 2016Article
- Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III.Nucleic acids research · 2015Article
- Gene duplication and neofunctionalization: POLR3G and POLR3GL.Genome research · 2014Article
- Male-dominant activation of rat renal organic anion transporter 1 (Oat1) and 3 (Oat3) expression by transcription factor BCL6.PloS one · 2012Article
- tRNA genes protect a reporter gene from epigenetic silencing in mouse cells.Cell cycle (Georgetown, Tex.) · 2011Article
- Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch.Genome research · 2011Article
- Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.Nature structural & molecular biology · 2011Article
- Atypical transcription of microRNA gene fragments.Nucleic acids research · 2010Article
- RNA polymerase III repression by the retinoblastoma tumor suppressor protein.Biochimica et biophysica actaReview
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Authors and funding
3 authors.
Funding
Abstract
RNA polymerase (pol) III contains a dissociable subcomplex that is required for initiation, but not for elongation or termination of transcription. This subcomplex is composed of subunits RPC3, RPC6 and RPC7, and interacts with TFIIIB, a factor that is necessary and sufficient to support accurate pol III transcription in vitro. Direct binding of TFIIIB to RPC6 is believed to recruit pol III to its genetic templates. However, this has never been tested in vivo. Here we combine chromatin immunoprecipitation with RNA interference to demonstrate that the RPC3/6/7 subcomplex is required for pol III recruitment in mammalian cells. Specific knockdown of RPC6 by RNAi results in post-transcriptional depletion of the other components of the subcomplex, RPC3 and RPC7, without destabilizing core pol III subunits or TFIIIB. The resultant core enzyme is defective in associating with TFIIIB and target genes in vivo. Promoter occupancy by pol II is unaffected, despite sharing five subunits with the pol III core. These observations provide evidence for the validity in vivo of the model for pol III recruitment that was built on biochemical data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.