ArticleApplied and environmental microbiology2008
Cyclic-di-GMP regulates extracellular polysaccharide production, biofilm formation, and rugose colony development by Vibrio vulnificus.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 97 citations in OpenAlex.
- Phylogeny-aware comparative genomics ofmBio · 2026Article
- The diguanylate cyclase and phosphodiesterase locally regulate the virulence factors in Vibrio vulnificus.Gut pathogens · 2025Article
- Identification of Three New Rugose Small Colony Variants from aMicroorganisms · 2025Article
- Biofilm Formation, c-di-GMP Production, and Antimicrobial Resistance in Staphylococcal Strains Isolated from Prosthetic Joint Infections: A Pilot Study in Total Hip and Knee Arthroplasty Patients.International journal of molecular sciences · 2025Article
- ARTP mutagenesis for genome-wide identification of genes important for biofilm regulation in spoilage bacteriumApplied and environmental microbiology · 2025Article
- Newly identified c-di-GMP pathway putative EAL domain gene STM0343 regulates stress resistance and virulence in Salmonella enterica serovar Typhimurium.Veterinary research · 2025Article
- Discovery of a Small-Molecule Inhibitor Targeting the Biofilm Regulator BrpT inJournal of microbiology and biotechnology · 2024Article
- Cyclic Diguanylate in the Wild: Roles During Plant and Animal Colonization.Annual review of microbiology · 2024Review
- Identification and characterization of a small molecule BFstatin inhibiting BrpR, the transcriptional regulator for biofilm formation ofFrontiers in microbiology · 2024Article
- Two novel genes identified by large-scale transcriptomic analysis are essential for biofilm and rugose colony development of Vibrio vulnificus.PLoS pathogens · 2023Article
- Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins inFrontiers in microbiology · 2023Article
- The PilT retraction ATPase promotes both extension and retraction of the MSHA type IVa pilus in Vibrio cholerae.PLoS genetics · 2022Article
- Virulence genes and antibiotic resistance profile ofVeterinary research forum : an international quarterly journal · 2022Article
- Nitric oxide stimulates type IV MSHA pilus retraction inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparative genome analyses of fiveMicrobial genomics · 2022Article
- Shigella flexneri Diguanylate Cyclases Regulate Virulence.Journal of bacteriology · 2021Article
- Identification of a Transcriptomic Network Underlying the Wrinkly and Smooth Phenotypes of Vibrio fischeri.Journal of bacteriology · 2021Article
- Transcriptomic Analysis Reveals That Municipal Wastewater Effluent EnhancesFrontiers in microbiology · 2021Article
- A Master Regulator BrpR Coordinates the Expression of Multiple Loci for Robust Biofilm and Rugose Colony Development inFrontiers in microbiology · 2021Article
- Virulence-associated genes and antibiotic resistance patterns of Vibrio spp. isolated from cultured marine fishes in Malaysia.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio vulnificus is a human and animal pathogen that carries the highest death rate of any food-borne disease agent. It colonizes shellfish and forms biofilms on the surfaces of plankton, algae, fish, and eels. Greater understanding of biofilm formation by the organism could provide insight into approaches to decrease its load in filter feeders and on biotic surfaces and control the occurrence of invasive disease. The capsular polysaccharide (CPS), although essential for virulence, is not required for biofilm formation under the conditions used here. In other bacteria, increased biofilm formation often correlates with increased exopolysaccharide (EPS) production. We exploited the translucent phenotype of acapsular mutants to screen a V. vulnificus genomic library and identify genes that imparted an opaque phenotype to both CPS biosynthesis and transport mutants. One of these encoded a diguanylate cyclase (DGC), an enzyme that synthesizes bis-(3'-5')-cyclic-di-GMP (c-di-GMP). This prompted us to use this DGC, DcpA, to examine the effect of elevated c-di-GMP levels on several developmental pathways in V. vulnificus. Increased c-di-GMP levels induced the production of an EPS that was distinct from the CPS and dramatically enhanced biofilm formation and rugosity in a CPS-independent manner. However, the EPS could not compensate for the loss of CPS production that is required for virulence. In contrast to V. cholerae, motility and virulence appeared unaffected by elevated levels of c-di-GMP.
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