ArticleJournal of bacteriology2008
Role of the global transcriptional regulator PrrA in Rhodobacter sphaeroides 2.4.1: combined transcriptome and proteome analysis.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Enhanced PrrAB system activation and restricted farnesyl pyrophosphate diversion underlie high coenzyme QSynthetic and systems biotechnology · 2026Article
- The blue light-dependent LOV-protein LdaP ofFrontiers in microbiology · 2024Article
- Antisense RNA asPcrL regulates expression of photosynthesis genes inRNA biology · 2021Article
- Membrane Sensor Histidine Kinases: Insights from Structural, Ligand and Inhibitor Studies of Full-Length Proteins and Signalling Domains for Antibiotic Discovery.Molecules (Basel, Switzerland) · 2021Review
- Effects of Light Regulation on Proteome Expression in Rhodobacter sphaeroides 2.4.1.Molecular biotechnology · 2021Article
- Article
- Use of transcriptomic data for extending a model of the AppA/PpsR system in Rhodobacter sphaeroides.BMC systems biology · 2017Article
- RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant ofFrontiers in cellular and infection microbiology · 2017Article
- Different Functions of Phylogenetically Distinct Bacterial Complex I Isozymes.Journal of bacteriology · 2016Article
- An integrated approach to reconstructing genome-scale transcriptional regulatory networks.PLoS computational biology · 2015Article
- The TyrR transcription factor regulates the divergent akr-ipdC operons of Enterobacter cloacae UW5.PloS one · 2015Article
- Global analysis of photosynthesis transcriptional regulatory networks.PLoS genetics · 2014Article
- Gene co-expression network analysis in Rhodobacter capsulatus and application to comparative expression analysis of Rhodobacter sphaeroides.BMC genomics · 2014Article
- Article
- Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy.Photosynthesis research · 2014Article
- A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.Nature structural & molecular biology · 2013Article
- RegA, the regulator of the two-component system RegB/RegA of Brucella suis, is a controller of both oxidative respiration and denitrification required for chronic infection in mice.Infection and immunity · 2013Article
- Life in an arsenic-containing gold mine: genome and physiology of the autotrophic arsenite-oxidizing bacterium rhizobium sp. NT-26.Genome biology and evolution · 2013Article
- Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Bacterial adaptation of respiration from oxic to microoxic and anoxic conditions: redox control.Antioxidants & redox signaling · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The PrrBA two-component regulatory system is a major global regulator in Rhodobacter sphaeroides 2.4.1. Here we have compared the transcriptome and proteome profiles of the wild-type (WT) and mutant PrrA2 cells grown anaerobically in the dark with dimethyl sulfoxide as an electron acceptor. Approximately 25% of the genes present in the PrrA2 genome are regulated by PrrA at the transcriptional level, either directly or indirectly, by twofold or more relative to the WT. The genes affected are widespread throughout all COG (cluster of orthologous group) functional categories, with previously unsuspected "metabolic" genes affected in PrrA2 cells. PrrA was found to act as both an activator and a repressor of transcription, with more genes being repressed in the presence of PrrA (9:5 ratio). An analysis of the genes encoding the 1,536 peptides detected through our chromatographic study, which corresponds to 36% coverage of the genome, revealed that approximately 20% of the genes encoding these proteins were positively regulated, whereas approximately 32% were negatively regulated by PrrA, which is in excellent agreement with the percentages obtained for the whole-genome transcriptome profile. In addition, comparison of the transcriptome and proteome mean parameter values for WT and PrrA2 cells showed good qualitative agreement, indicating that transcript regulation paralleled the corresponding protein abundance, although not one for one. The microarray analysis was validated by direct mRNA measurement of randomly selected genes that were both positively and negatively regulated. lacZ transcriptional and kan translational fusions enabled us to map putative PrrA binding sites and revealed potential gene targets for indirect regulation by PrrA.
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