ArticleJournal of bacteriology2008
Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Horizontal Gene Transfer of Genes Encoding Copper-Containing Membrane-Bound Monooxygenase (CuMMO) and Soluble Di-iron Monooxygenase (SDIMO) in Ethane- and Propane-OxidizingApplied and environmental microbiology · 2021Article
- Virulence Plasmids of the Pathogenic Clostridia.Microbiology spectrum · 2019Article
- Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3.Nature communications · 2018Article
- Critical Components of the Conjugation Machinery of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.Journal of bacteriology · 2015Article
- Clostridium perfringens type A-E toxin plasmids.Research in microbiology · 2015Review
- Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens.Journal of bacteriology · 2015Article
- Virulence Plasmids of Spore-Forming Bacteria.Microbiology spectrum · 2014Review
- Delineation of polar localization domains of Agrobacterium tumefaciens type IV secretion apparatus proteins VirB4 and VirB11.MicrobiologyOpen · 2014Article
- Key components of the eight classes of type IV secretion systems involved in bacterial conjugation or protein secretion.Nucleic acids research · 2014Article
- Review
- Toxin plasmids of Clostridium perfringens.Microbiology and molecular biology reviews : MMBR · 2013Review
- Evolution of conjugation and type IV secretion systems.Molecular biology and evolution · 2013Article
- The stb operon balances the requirements for vegetative stability and conjugative transfer of plasmid R388.PLoS genetics · 2011Article
- Article
- Article
- Membrane-associated DNA transport machines.Cold Spring Harbor perspectives in biology · 2010Review
- Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.Journal of bacteriology · 2010Article
- Conjugative transfer of the integrative and conjugative element ICEBs1 from Bacillus subtilis likely initiates at the donor cell pole.Journal of bacteriology · 2010Article
- Biological diversity of prokaryotic type IV secretion systems.Microbiology and molecular biology reviews : MMBR · 2009Review
- The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex.Journal of bacteriology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
In Clostridium perfringens, conjugative plasmids encode important virulence factors, such as toxins and resistance determinants. All of these plasmids carry a conjugation locus that consists of 11 genes: intP and tcpA to tcpJ. Three proteins, TcpA, a potential coupling protein, TcpF, a putative ATPase that is similar to ORF15 from Tn916, and TcpH, which contains VirB6-like domains, are essential for conjugation in the prototype conjugative plasmid pCW3. To analyze the functional domains of TcpH, a putative structural component of the mating-pair formation complex and deletion and site-directed mutants were constructed and analyzed. The results showed that the N-terminal 581 residues and the conserved (242)VQQPW(246) motif were required for conjugative transfer. Bacterial two-hybrid and biochemical studies showed that TcpH interacted with itself and with TcpC. An analysis of the tcpH mutants demonstrated that the region required for these interactions also was localized to the N-terminal 581 residues and that the function of the C-terminal region of TcpH was independent of protein-protein interactions. Finally, immunofluorescence studies showed that TcpH and TcpF were located at both cell poles of donor C. perfringens cells. The results provide evidence that TcpH is located in the cell membrane, where it oligomerizes and interacts with TcpC to form part of the mating-pair formation complex, which is located at the cell poles and is closely associated with TcpF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.