ArticleJournal of bacteriology2008
Symbiosis-promoting and deleterious effects of NopT, a novel type 3 effector of Rhizobium sp. strain NGR234.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 110 citations in OpenAlex.
- Type III effectors of symbiotic Rhizobia include diverse predicted transcriptional and post-transcriptional modulators.PLoS biology · 2026Article
- Type III secretion system diversity in the Pseudomonas fluorescens species complex.The New phytologist · 2026Article
- Comparative Genomic Analysis of the Type VI Secretion System in Strains of the Rhizobium leguminosarum Species Complex with Variable Host Specificity.Current microbiology · 2026Article
- Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotusjaponicus.Plant communications · 2026Article
- Diversity of bradyrhizobial T3SS systems and their roles in symbiosis with peanut (Applied and environmental microbiology · 2025Article
- Article
- NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.Scientific reports · 2024Article
- Broad-spectrum ubiquitin/ubiquitin-like deconjugation activity of the rhizobial effector NopD from Bradyrhizobium (sp. XS1150).Communications biology · 2024Article
- NopAA and NopD Signaling Association-Related GeneInternational journal of molecular sciences · 2023Article
- NopC/T/L Signal Crosstalk GeneInternational journal of molecular sciences · 2023Article
- The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis.International journal of molecular sciences · 2022Review
- Friend or foe: How plants discriminate between pathogenic and mutualistic bacteria.Plant physiology · 2022Article
- Effector-triggered inhibition of nodulation: A rhizobial effector protease targets soybean kinase GmPBS1-1.Plant physiology · 2022Article
- Bridging the Gap: Type III Secretion Systems in Plant-Beneficial Bacteria.Microorganisms · 2022Article
- Article
- Host manipulation by bacterial type III and type IV secretion system effector proteases.Cellular microbiology · 2021Review
- Bacterial effectors mimicking ubiquitin-proteasome pathway tweak plant immunity.Microbiological research · 2021Review
- Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus.Plant methods · 2021Article
- Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species.Scientific reports · 2021Article
- Genomic Diversity of Pigeon Pea (Frontiers in plant science · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Establishment of symbiosis between certain host plants and nitrogen-fixing bacteria ("rhizobia") depends on type 3 effector proteins secreted via the bacterial type 3 secretion system (T3SS). Here, we report that the open reading frame y4zC of strain NGR234 encodes a novel rhizobial type 3 effector, termed NopT (for nodulation outer protein T). Analysis of secreted proteins from NGR234 and T3SS mutants revealed that NopT is secreted via the T3SS. NopT possessed autoproteolytic activity when expressed in Escherichia coli or human HEK 293T cells. The processed NopT exposed a glycine (G50) to the N terminus, which is predicted to be myristoylated in eukaryotic cells. NopT with a point mutation at position C93, H205, or D220 (catalytic triad) showed strongly reduced autoproteolytic activity, indicating that NopT is a functional protease of the YopT-AvrPphB effector family. When transiently expressed in tobacco plants, proteolytically active NopT elicited a rapid hypersensitive reaction. Arabidopsis plants transformed with nopT showed chlorotic and necrotic symptoms, indicating a cytotoxic effect. Inoculation experiments with mutant derivatives of NGR234 indicated that NopT affected nodulation either positively (Phaseolus vulgaris cv. Yudou No. 1; Tephrosia vogelii) or negatively (Crotalaria juncea). We suggest that NopT-related polymorphism may be involved in evolutionary adaptation of NGR234 to particular host legumes.
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