ArticleBiophysical journal2008
Mucus secretion and cytoskeletal modifications in cultured nasal epithelial cells exposed to wall shear stresses.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- A Dynamic Breathing Lung Chip for Precise Evaluation of Inhaled Drug Efficacy and Airway Epithelial Responses.ACS biomaterials science & engineering · 2025Article
- A Cell-Based Nasal Model for Screening the Deposition, Biocompatibility, and Transport of Aerosolized PLGA Nanoparticles.Molecular pharmaceutics · 2024Article
- The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 2-Advanced Microfluidic Nasal In Vitro Model for Drug Absorption Testing.Pharmaceutics · 2023Article
- The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 1-Establishing a Nasal In Vitro Model for Drug Delivery Testing Based on a Novel Cell Line.Pharmaceutics · 2023Article
- Transmembrane Mucin Response in Conjunctival Epithelial Cells Exposed to Wall Shear Stresses.International journal of molecular sciences · 2023Article
- Numerical modelling of micron particle inhalation in a realistic nasal airway with pediatric adenoid hypertrophy: A virtual comparison between pre- and postoperative models.Frontiers in pediatrics · 2023Article
- 3D printed transwell-integrated nose-on-chip model to evaluate effects of air flow-induced mechanical stresses on mucous secretion.Biomedical microdevices · 2022Article
- Antioxidant Effect and Acute Oral Toxicity of Hot Springs.Computational intelligence and neuroscience · 2022Article
- Review
- Prediction of upper airway dryness and optimal continuous positive airway pressure conditions.Journal of biomechanics · 2020Article
- Effect of continuous positive airway pressure treatment on permeability, inflammation and mucus production of human epithelial cells.ERJ open research · 2020Article
- Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability.PloS one · 2018Article
- Simvastatin Treatment Modulates Mechanically-Induced Injury and Inflammation in Respiratory Epithelial Cells.Annals of biomedical engineering · 2016Article
- Article
- A microfluidic model to study fluid dynamics of mucus plug rupture in small lung airways.Biomicrofluidics · 2015Article
- A microfluidic device to apply shear stresses to polarizing ciliated airway epithelium using air flow.Biomicrofluidics · 2014Article
- Asthmatic and normal respiratory epithelial cells respond differently to mechanical apical stress.American journal of respiratory and critical care medicine · 2014Article
- Fluid-flow induced wall shear stress and epithelial ovarian cancer peritoneal spreading.PloS one · 2013Article
- Mechanophysical stimulations of mucin secretion in cultures of nasal epithelial cells.Biophysical journal · 2011Article
- Proteomics of buccal cavity mucus in female tilapia fish (Oreochromis spp.): a comparison between parental and non-parental fish.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nasal epithelium is continuously subjected to wall shear stresses (WSS) induced by respiratory airflows. An in vitro experimental model was developed to expose nasal epithelial cells cultured under air-liquid interface conditions to steady airflow-induced WSS. Mucus secretion from epithelial goblet cells was quantified using an enzyme-linked lectinosorbent assay, and modifications of the cytoskeletal structure were qualitatively evaluated from fluorescent stains of actin and beta-tubulin fibers. The results show increased mucus secretion from cells subjected to WSS of 0.1 and 1.0 dyne/cm(2) for more than 15 min in comparison with unstressed cells. The integrity levels of beta-tubulin fibers were significantly lower in cells subjected to WSS than in unstressed cells. The increased mucus secretion in response to WSS was approximately the same in Taxol-free and Taxol-treated cultures, which indicates that there is no direct connection between beta-tubulin fragmentation and mucus secretion. The stressed cells regained their normal cytoskeletal appearance 24 h after the exposure to WSS. The results of this study suggest that WSS have an important role in the mechanical regulation of the nasal surface epithelium function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.