ArticleMolecular and cellular biology1991
Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- Quantitative RNA-seq meta-analysis of alternative exon usage inGenome research · 2017Pooled it
- Genome annotation of Caenorhabditis briggsae by TEC-RED identifies new exons, paralogs, and conserved and novel operons.G3 (Bethesda, Md.) · 2022Article
- Evidence of constraint in the 3D genome for trans-splicing in human cells.Science China. Life sciences · 2020Article
- Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni.Scientific reports · 2018Article
- The transcription start site landscape of C. elegans.Genome research · 2013Article
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Regulatory elements of Caenorhabditis elegans ribosomal protein genes.BMC genomics · 2012Article
- The Caenorhabditis elegans intermediate-size transcriptome shows high degree of stage-specific expression.Nucleic acids research · 2011Article
- Core promoter T-blocks correlate with gene expression levels in C. elegans.Genome research · 2011Article
- Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene.Nucleic acids research · 2011Article
- A global analysis of C. elegans trans-splicing.Genome research · 2011Article
- Identification of transcription start sites of trans-spliced genes: uncovering unusual operon arrangements.RNA (New York, N.Y.) · 2011Article
- Article
- Polycistronic pre-mRNA processing in vitro: snRNP and pre-mRNA role reversal in trans-splicing.Genes & development · 2010Article
- High-throughput sequence analysis of Ciona intestinalis SL trans-spliced mRNAs: alternative expression modes and gene function correlates.Genome research · 2010Article
- The splice leader addition domain represents an essential conserved motif for heterologous gene expression in B. malayi.Molecular and biochemical parasitology · 2009Article
- Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders.RNA (New York, N.Y.) · 2008Article
- A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans.Molecular genetics and genomics : MGG · 2004Article
- Genome annotation by high-throughput 5' RNA end determination.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- An uncapped RNA suggests a model for Caenorhabditis elegans polycistronic pre-mRNA processing.RNA (New York, N.Y.) · 2003Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
In nematodes, the RNA products of some genes are trans-spliced to a 22-nucleotide spliced leader (SL), while the RNA products of other genes are not. In Caenorhabditis elegans, there are two SLs, SL1 and SL2, donated by two distinct small nuclear ribonucleoprotein particles in a process functionally quite similar to nuclear intron removal. We demonstrate here that it is possible to convert a non-trans-spliced gene into a trans-spliced gene by placement of an intron missing only the 5' splice site into the 5' untranslated region. Stable transgenic strains were isolated expressing a gene in which 69 nucleotides of a vit-5 intron, including the 3' splice site, were inserted into the 5' untranslated region of a vit-2/vit-6 fusion gene. The RNA product of this gene was examined by primer extension and PCR amplification. Although the vit-2/vit-6 transgene product is not normally trans-spliced, the majority of transcripts from this altered gene were trans-spliced to SL1. We termed the region of a trans-spliced mRNA precursor between the 5' end and the first 3' splice site an "outron." Our results suggest that if a transcript begins with intronlike sequence followed by a 3' splice site, this alone may constitute an outron and be sufficient to demarcate a transcript as a trans-splice acceptor. These findings leave open the possibility that specific sequences are required to increase the efficiency of trans-splicing.
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