ArticleMolecular and cellular biology1991
Retroviral insertions 90 kilobases proximal to the Evi-1 myeloid transforming gene activate transcription from the normal promoter.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo.The Journal of clinical investigation · 2007Trial
- Alpharetroviral vectors: from a cancer-causing agent to a useful tool for human gene therapy.Viruses · 2014Review
- Correction of murine SCID-X1 by lentiviral gene therapy using a codon-optimized IL2RG gene and minimal pretransplant conditioning.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- The first sequenced carnivore genome shows complex host-endogenous retrovirus relationships.PloS one · 2011Article
- A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors.BMC biotechnology · 2009Article
- Murine leukemias with retroviral insertions at Lmo2 are predictive of the leukemias induced in SCID-X1 patients following retroviral gene therapy.PLoS genetics · 2009Article
- Evi1 is a survival factor which conveys resistance to both TGFbeta- and taxol-mediated cell death via PI3K/AKT.Oncogene · 2006Article
- Animal models of acute myelogenous leukaemia - development, application and future perspectives.Leukemia · 2005Review
- The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery.Genetic vaccines and therapy · 2004Article
- The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E.The Journal of cell biology · 2004Article
- The common retroviral insertion locus Dsi1 maps 30 kilobases upstream of the P1 promoter of the murine Runx3/Cbfa3/Aml2 gene.Journal of virology · 2002Article
- Genetic heterogeneity in acute myeloid leukemia: maximizing information flow from MuLV mutagenesis studies.Leukemia · 2000Review
- Activation of Hex and mEg5 by retroviral insertion may contribute to mouse B-cell leukemia.Oncogene · 1999Article
- Article
- Intergenic splicing of MDS1 and EVI1 occurs in normal tissues as well as in myeloid leukemia and produces a new member of the PR domain family.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Dual functions of the AML1/Evi-1 chimeric protein in the mechanism of leukemogenesis in t(3;21) leukemias.Molecular and cellular biology · 1995Article
- Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- PU.1 and an HLH family member contribute to the myeloid-specific transcription of the Fc gamma RIIIA promoter.The EMBO journal · 1994Article
- Evidence for long-range oncogene activation by hepadnavirus insertion.The EMBO journal · 1994Article
- Consistent intergenic splicing and production of multiple transcripts between AML1 at 21q22 and unrelated genes at 3q26 in (3;21)(q26;q22) translocations.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The inappropriate production of the Evi-1 zinc finger protein occurs in retrovirus-induced murine myeloid leukemias and human acute myelogenous leukemias. In murine leukemias, expression of the Evi-1 gene is associated with retroviral insertions either in the Evi-1 locus, which is immediately 5' of the coding region of the gene, or in the genetically linked Cb-1/fim-3 locus. In these studies, we demonstrate by chromosomal walking and pulse field electrophoresis that the Cb-1/fim-3 locus is located 90 kb 5' of the Evi-1 locus. Primary structure analysis of Evi-1 cDNA clones from a Cb-1/fim-3 rearranged cell line (DA-3) demonstrates that transcription initiates 5' of the Evi-1 locus and that the first noncoding exon of the gene is 681 bp larger than previously defined. S1 nuclease protection studies reveal multiple transcription initiation sites within this region. Comparable transcriptional initiation sites were identified in RNA from kidney and ovary, in which the gene is normally expressed, suggesting that retroviral insertions in the Cb-1/fim-3 locus activate transcription from the normal promoter. In one myeloid cell line (DA-3), a single long terminal repeat (LTR) is present in the Cb-1/fim-3 locus. No stable transcripts were detectable from this LTR. In cells with retroviral insertions in the Cb-1/fim-3 locus, one allele of the Evi-1 locus becomes hypermethylated in the 5' region of the gene. Together, these results are most consistent with an LTR-mediated, long-range cis activation of Evi-1 gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.