ArticleDevelopmental biology2008
Lis1/dynactin regulates metaphase spindle orientation in Drosophila neuroblasts.
Article in Developmental biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 96 citations in OpenAlex.
- Microtubule pushing and cortical pulling cooperate to orient and position the mitotic spindle.The EMBO journal · 2026Article
- Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains.Journal of visualized experiments : JoVE · 2023Article
- Article
- Diversity of activator of G-protein signaling (AGS)-family proteins and their impact on asymmetric cell division across taxa.Developmental biology · 2020Review
- Development and Patterning of the Cochlea: From Convergent Extension to Planar Polarity.Cold Spring Harbor perspectives in medicine · 2020Review
- An Immobilization Technique for Long-Term Time-Lapse Imaging of ExplantedFrontiers in cell and developmental biology · 2020Article
- Fighting Cancer Stem Cell Fate by Targeting LIS1 a WD40 Repeat Protein.Frontiers in oncology · 2019Article
- Emerging mechanisms of asymmetric stem cell division.The Journal of cell biology · 2018Review
- Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity inThe EMBO journal · 2018Article
- The Role of WD40-Repeat Protein 62 (MCPH2) in Brain Growth: Diverse Molecular and Cellular Mechanisms Required for Cortical Development.Molecular neurobiology · 2018Review
- A CRISPR Tagging-Based Screen Reveals Localized Players in Wnt-Directed Asymmetric Cell Division.Genetics · 2018Article
- Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity.Molecular biology of the cell · 2017Article
- Dynein is regulated by the stability of its microtubule track.The Journal of cell biology · 2017Article
- Neuroblast niche position is controlled by Phosphoinositide 3-kinase-dependent DE-Cadherin adhesion.Development (Cambridge, England) · 2017Article
- Dynactin has two antagonistic regulatory domains and exerts opposing effects on dynein motility.PloS one · 2017Article
- Preferential Association of Lissencephaly-1 Gene Expression with CD133+ Glioblastoma Cells.Journal of Cancer · 2017Article
- Coupling changes in cell shape to chromosome segregation.Nature reviews. Molecular cell biology · 2016Review
- Cell Fate Decision Making through Oriented Cell Division.Journal of developmental biology · 2015Article
- Genes and brain malformations associated with abnormal neuron positioning.Molecular brain · 2015Review
- Cytoskeletal dynamics: a view from the membrane.The Journal of cell biology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mitotic spindle orientation in polarized cells determines whether they divide symmetrically or asymmetrically. Moreover, regulated spindle orientation may be important for embryonic development, stem cell biology, and tumor growth. Drosophila neuroblasts align their spindle along an apical/basal cortical polarity axis to self-renew an apical neuroblast and generate a basal differentiating cell. It is unknown whether spindle alignment requires both apical and basal cues, nor have molecular motors been identified that regulate spindle movement. Using live imaging of neuroblasts within intact larval brains, we detect independent movement of both apical and basal spindle poles, suggesting that forces act on both poles. We show that reducing astral microtubules decreases the frequency of spindle movement, but not its maximum velocity, suggesting that one or few microtubules can move the spindle. Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces. Loss of either astral microtubules or Lis1/dynactin leads to spindle/cortical polarity alignment defects at metaphase, but these are rescued by telophase. We propose that an early Lis1/dynactin-dependent pathway and a late Lis1/dynactin-independent pathway regulate neuroblast spindle orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.