ArticleMolecular microbiology2008
A basic/hydrophobic cleft of the T4 activator MotA interacts with the C-terminus of E.coli sigma70 to activate middle gene transcription.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 24 citations in OpenAlex.
- Transcriptional reprogramming by bacteriophage T4: turning the host transcriptional machinery to the dark side.EcoSal Plus · 2025Review
- Tevenvirinae phages encode a family of LSm-like fold proteins.Computational and structural biotechnology journal · 2025Article
- Setting Up a Better Infection: Overexpression of the Early Bacteriophage T4 GenePHAGE (New Rochelle, N.Y.) · 2022Article
- A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing.Nucleic acids research · 2021Article
- Overexpression of the Bacteriophage T4Viruses · 2021Article
- Structural basis of σ appropriation.Nucleic acids research · 2019Article
- A Novel Bvg-Repressed Promoter CausesJournal of bacteriology · 2018Article
- Article
- The phage T4 MotA transcription factor contains a novel DNA binding motif that specifically recognizes modified DNA.Nucleic acids research · 2018Article
- A 3D puzzle approach to building protein-DNA structures.Transcription · 2017Article
- Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase.Nucleic acids research · 2016Article
- Architecture of the bacteriophage T4 activator MotA/promoter DNA interaction during sigma appropriation.The Journal of biological chemistry · 2013Article
- The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA.Microbiology (Reading, England) · 2012Review
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- Different requirements for σ Region 4 in BvgA activation of the Bordetella pertussis promoters P(fim3) and P(fhaB).Journal of molecular biology · 2011Article
- A mutation within the β subunit of Escherichia coli RNA polymerase impairs transcription from bacteriophage T4 middle promoters.Journal of bacteriology · 2010Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression.Molecular microbiology · 2009Article
- The secret to 6S: regulating RNA polymerase by ribo-sequestration.Molecular microbiology · 2009Article
- Inhibition of transcription in Staphylococcus aureus by a primary sigma factor-binding polypeptide from phage G1.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Transcriptional activation often employs a direct interaction between an activator and RNA polymerase. For activation of its middle genes, bacteriophage T4 appropriates Escherichia coli RNA polymerase through the action of two phage-encoded proteins, MotA and AsiA. Alone, AsiA inhibits transcription from a large class of host promoters by structurally remodelling region 4 of sigma(70), the primary specificity subunit of E. coli RNA polymerase. MotA interacts both with sigma(70) region 4 and with a DNA element present in T4 middle promoters. AsiA-induced remodelling is proposed to make the far C-terminus of sigma(70) region 4 accessible for MotA binding. Here, NMR chemical shift analysis indicates that MotA uses a 'basic/hydrophobic' cleft to interact with the C-terminus of AsiA-remodelled sigma(70), but MotA does not interact with AsiA itself. Mutations within this cleft, at residues K3, K28 and Q76, both impair the interaction of MotA with sigma(70) region 4 and MotA-dependent activation. Furthermore, mutations at these residues greatly decrease phage viability. Most previously described activators that target sigma(70) directly use acidic residues to engage a basic surface of region 4. Our work supports accumulated evidence indicating that 'sigma appropriation' by MotA and AsiA uses a fundamentally different mechanism to activate transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.