ArticleThe Plant journal : for cell and molecular biology2008
If the antibody fails--a mass western approach.
Article in The Plant journal : for cell and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- PANOMICS meets germplasm.Plant biotechnology journal · 2020Review
- Subcellular Phenotyping: Using Proteomics to Quantitatively Link Subcellular Leaf Protein and Organelle Distribution Analyses ofFrontiers in plant science · 2019Article
- Multiple marker abundance profiling: combining selected reaction monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples.The Plant journal : for cell and molecular biology · 2017Article
- Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks.Journal of experimental botany · 2017Article
- Dynamic Acclimation to High Light inFrontiers in plant science · 2017Article
- A Proteomic View on the Role of Legume Symbiotic Interactions.Frontiers in plant science · 2017Review
- Article
- Beyond the Western front: targeted proteomics and organelle abundance profiling.Frontiers in plant science · 2015Article
- Discordance between protein and transcript levels detected by selected reaction monitoring.Plant signaling & behavior · 2015Article
- The role of proteomics in progressing insights into plant secondary metabolism.Frontiers in plant science · 2015Review
- Loss of the two major leaf isoforms of sucrose-phosphate synthase in Arabidopsis thaliana limits sucrose synthesis and nocturnal starch degradation but does not alter carbon partitioning during photosynthesis.Journal of experimental botany · 2014Article
- Selected reaction monitoring to determine protein abundance in Arabidopsis using the Arabidopsis proteotypic predictor.Plant physiology · 2014Article
- Mathematical modeling reveals that metabolic feedback regulation of SnRK1 and hexokinase is sufficient to control sugar homeostasis from energy depletion to full recovery.Frontiers in plant science · 2014Article
- Field-omics-understanding large-scale molecular data from field crops.Frontiers in plant science · 2014Article
- ABI1 and PP2CA phosphatases are negative regulators of Snf1-related protein kinase1 signaling in Arabidopsis.The Plant cell · 2013Article
- MASCP Gator: an aggregation portal for the visualization of Arabidopsis proteomics data.Plant physiology · 2011Article
- A label-free mass spectrometry method for the quantification of protein isotypes.Analytical biochemistry · 2009Article
- Identification of L-ferritin in neuromelanin granules of the human substantia nigra: a targeted proteomics approach.Molecular & cellular proteomics : MCP · 2009Article
- Absolute quantification of Medicago truncatula sucrose synthase isoforms and N-metabolism enzymes in symbiotic root nodules and the detection of novel nodule phosphoproteins by mass spectrometry.Journal of experimental botany · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
summarySucrose-phosphate synthase (SPS) has attracted the interest of plant scientists for decades. It is the key enzyme in sucrose metabolism and is under investigation in various plant species, e.g. spinach, tobacco, poplar, resurrection plants, maize, rice, kiwi and Arabidopsis thaliana. In A. thaliana, there are four distinct SPS isoforms. Their expression is thought to depend on environmental conditions and plant tissue. However, these data were derived from mRNA expression levels only. No data on SPS protein identification from crude extracts have been available until now. An antibody approach failed to distinguish the four isoforms. Therefore, we developed a method for SPS quantification and isoform-specific identification in A. thaliana complex protein samples. Samples were separated on SDS-PAGE, digested and directly applied to liquid chromatography/triple-stage quadrupole mass spectrometry (LC/TSQ-MS). In this approach, known as mass Western, samples were analysed in multi-reaction monitoring (MRM) mode, so that all four SPS isoforms could be measured in one experiment. In addition to the relative quantification, stable isotope-labelled internal peptide standards allowed absolute quantification of SPS proteins. Protein extracts from various plant tissues, samples harvested during the day or the night, and cold-stressed plants were analysed. The stress-specific SPS5a isoform showed increased concentrations in cold-stressed leaf material.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.