ArticleEndocrinology2008
Kisspeptin excites gonadotropin-releasing hormone neurons through a phospholipase C/calcium-dependent pathway regulating multiple ion channels.
Article in Endocrinology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
115 citing papers in PubMed, 2 syntheses or guidelines pooled it, 266 citations in OpenAlex.
- Pooled it
- Kisspeptins in human reproduction-future therapeutic potential.Journal of assisted reproduction and genetics · 2012Pooled it
- Kisspeptin receptor agonist has therapeutic potential for female reproductive disorders.The Journal of clinical investigation · 2020Trial
- Article
- Knockout of PI4-Kinase A in GnRH Neurons Causes their Prepubertal Death.bioRxiv : the preprint server for biology · 2026Article
- Exploring the Role of Kisspeptin in Polycystic Ovary Syndrome and Its Associated Pregnancy Complications.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2026Review
- PI4-kinase A is essential for survival of the GnRH neurons.Frontiers in endocrinology · 2026Article
- Comprehensive chemoanatomical mapping, and the gonadal regulation, of seven kisspeptin neuronal populations in the mouse brain.Journal of neuroendocrinology · 2025Article
- The kisspeptin system in and beyond reproduction: exploring intricate pathways and potential links between endometriosis and polycystic ovary syndrome.Reviews in endocrine & metabolic disorders · 2024Review
- Reappraisal of the fundamental mechanisms of the sHA14-1 molecule as a Bcl-2/Bcl-XL ligand in the context of anticancer therapy: A cell biological study.Journal of biological methods · 2024Article
- Gonadal Feedback Alters the Relationship between Action Potentials and Hormone Release in Gonadotropin-Releasing Hormone Neurons in Male Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Mechanisms linking neurological disorders with reproductive endocrine dysfunction: Insights from epilepsy research.Frontiers in neuroendocrinology · 2023Review
- Effect of CPP-related genes on GnRH secretion and Notch signaling pathway during puberty.Biomedical journal · 2023Review
- Modelling KNDy neurons and gonadotropin-releasing hormone pulse generation.Current opinion in endocrine and metabolic research · 2022Review
- The roles of GnRH in the human central nervous system.Hormones and behavior · 2022Review
- Lack of Oestrogen Receptor Expression in Breast Cancer Cells Does Not Correlate with Kisspeptin Signalling and Migration.International journal of molecular sciences · 2022Article
- Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats.International journal of molecular sciences · 2022Article
- The Role of Kisspeptin in the Pathogenesis of Pregnancy Complications: A Narrative Review.International journal of molecular sciences · 2022Review
- Estrogenic regulation of reproduction and energy homeostasis by a triumvirate of hypothalamic arcuate neurons.Journal of neuroendocrinology · 2022Review
- Mouse Cre drivers: tools for studying disorders of the human female neuroendocrine-reproductive axis†.Biology of reproduction · 2022Review
55 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The present study used perforated-patch electrophysiology and calcium imaging in GnRH transgenic mouse lines to determine the mechanisms underlying the potent excitatory effects of kisspeptin upon GnRH neurons in the acute brain slice preparation. Kisspeptin (100 nm) depolarized (6 +/- 1 mV) and/or evoked an 87 +/- 4% increase in firing rate of 75% of adult GnRH neurons (n = 51). No sex differences were found. Analyses of input resistance and current-voltage curves indicated that a heterogeneous closure of potassium channels and opening of nonselective cation (NSC) channels was involved in kisspeptin's depolarizing response. Pharmacological pretreatment with either barium, a potassium channel blocker, or flufenamic acid, an NSC channel antagonist, reduced the percentage of responding GnRH neurons from 75 to 40% (P < 0.05). Pretreatment with both barium and flufenamic acid reduced the response rate to 17% (P < 0.05). To examine the intracellular signaling cascade involved, GnRH neurons were treated with antagonists of phospholipase C (PLC), inositol-trisphosphate receptors (IP3R), and ERK1/2 before kisspeptin exposure. PLC and IP3R antagonism reduced the percentage of responding GnRH neurons from 80 to 15 and 7%, respectively (P < 0.001). Real-time calcium imaging showed that kisspeptin evoked an approximately 10% increase in intracellular calcium levels in GnRH neurons that was followed by a decrease and return to pretest calcium levels. Additional experiments indicated that mechanisms intrinsic to the GnRH neuron are responsible for their prolonged depolarizing response to kisspeptin. These studies indicate that kisspeptin activates G protein-coupled receptor 54 (GPR54) to initiate a PLC-IP3R-calcium cascade that modulates both potassium and NSC channels to initiate depolarization in GnRH neurons.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.