ArticleMolecular imaging and biology
Direct site-specific radiolabeling of an Affibody protein with 4-[18F]fluorobenzaldehyde via oxime chemistry.
Article in Molecular imaging and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Innovative Peptide Bioconjugation Chemistry with Radionuclides: Beyond Classical Click Chemistry.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Imaging using radiolabelled targeted proteins: radioimmunodetection and beyond.EJNMMI radiopharmacy and chemistry · 2020Review
- Affibody Molecules as Targeting Vectors for PET Imaging.Cancers · 2020Review
- PET Imaging of HER2-Positive Tumors with Cu-64-Labeled Affibody Molecules.Molecular imaging and biology · 2019Article
- Development & automation of a novel [(18)F]F prosthetic group, 2-[(18)F]-fluoro-3-pyridinecarboxaldehyde, and its application to an amino(oxy)-functionalised Aβ peptide.Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine · 2016Article
- Site-Specific Radiofluorination of Biomolecules with 8-[(18)F]-Fluorooctanoic Acid Catalyzed by Lipoic Acid Ligase.ACS chemical biology · 2016Article
- Site-Specific Radioiodination of HER2-Targeting Affibody Molecules using 4-Iodophenethylmaleimide Decreases Renal Uptake of Radioactivity.ChemistryOpen · 2015Article
- Molecular imaging of EGFR/HER2 cancer biomarkers by protein MRI contrast agents.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Review
- Enhanced aqueous Suzuki-Miyaura coupling allows site-specific polypeptide 18F-labeling.Journal of the American Chemical Society · 2013Article
- Efficient method for site-specific 18F-labeling of biomolecules using the rapid condensation reaction between 2-cyanobenzothiazole and cysteine.Bioconjugate chemistry · 2012Article
- PET of EGFR expression with an 18F-labeled affibody molecule.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2012Article
- A novel 18F-labeled two-helix scaffold protein for PET imaging of HER2-positive tumor.European journal of nuclear medicine and molecular imaging · 2011Article
- Preparation of 18F-labeled peptides using the copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition.Nature protocols · 2011Article
- New F-18 prosthetic group via oxime coupling.Bioconjugate chemistry · 2011Article
- 64Cu-labeled affibody molecules for imaging of HER2 expressing tumors.Molecular imaging and biology · 2010Article
- Positive progress in immunoPET--not just a coincidence.Cancer biotherapy & radiopharmaceuticals · 2010Article
- Modular strategies for PET imaging agents.Current opinion in chemical biology · 2010Review
- A 2-helix small protein labeled with 68Ga for PET imaging of HER2 expression.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2009Article
- A novel method for direct site-specific radiolabeling of peptides using [18F]FDG.Bioconjugate chemistry · 2009Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
purposeIn this study, we introduce a methodology for preparing 18F-labeled Affibody protein, specifically 18F-Anti-HER2 dimeric Affibody (14 kDa), for in vivo imaging of HER2neu with positron emission tomography (PET). PROCEDURES: We have used 4-[18F]fluorobenzaldehyde as a synthon to prepare 18F-Anti-HER2 Affibody. Aminooxy-functionalized Affibody (Anti-HER2-ONH2) was incubated with 4-[18F]fluorobenzaldehyde in ammonium acetate buffer at pH 4 in the presence of methanol at 70 degrees C for 15 min. The resulting 18F-labeled Affibody molecule was evaluated as a PET probe in xenograft models expressing HER2.
resultsWe have successfully prepared 18F-Anti-HER2 dimeric Affibody (14 kDa), N-(4-[18F]fluorobenzylidine)oxime-Anti-HER2 Affibody, [18F]FBO-Anti-HER2, in 26-30% radiochemical yields (decay corrected). High-contrast small-animal PET images with relatively moderate tumor uptake (1.79 +/- 0.40% ID/g) were observed for the 18F-Anti-HER2 Affibody.
conclusionSite-specific 18F-labeled Affibody against HER2 has been synthesized via chemoselective oxime formation between an aminooxy-functionalized Affibody and 18F-fluorobenzaldehyde. The results have implications for radiolabeling of other affibodies and macromolecules and should also be important for advancing Affibody imaging with PET.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.