ArticleThe European physical journal. E, Soft matter
Key role of receptor density in colloid/cell specific interaction: a quantitative biomimetic study on giant vesicles.
Article in The European physical journal. E, Soft matter. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Article
- Review
- The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization.BMC biology · 2021Article
- Modeling Native EHEC Outer Membrane Vesicles by Creating Synthetic Surrogates.Microorganisms · 2020Article
- Whole-GUV patch-clamping.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Physical Principles of Nanoparticle Cellular Endocytosis.ACS nano · 2015Review
- Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid.PloS one · 2013Article
- Article
- Article
- Entropy-driven aggregation of adhesion sites of supported membranes.The European physical journal. E, Soft matter · 2010Article
- A simple method for the reconstitution of membrane proteins into giant unilamellar vesicles.The Journal of membrane biology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper presents an experimental study of the adsorption of colloids on model membranes mediated by specific ligand-receptor interactions. The colloids consist of lipid multilamellar liposomes (spherulites) functionalized with the B-subunit of Shiga Toxin (STxB), while the membranes are lipid Giant Unilamellar Vesicles (GUV) containing STxB lipid receptor, Globotriaosylceramide (Gb3). Through confocal microscopy and flow cytometry, we show the specificity of the adsorption. Moreover, we show that flow cytometry can be used to efficiently quantify the kinetics of colloid adsorption on GUVs with very good statistics. By varying the bulk colloid concentration and receptor density in the membrane, we point out the existence of an optimum Gb3 density for adsorption. We propose that this optimum corresponds to a transition between reversible colloid adsorption at low Gb3 density and irreversible adsorption, and likely spherulite fusion, at high density. We compare our results both to STxB-colloids adhering on living cells and to free STxB proteins interacting with GUVs containing Gb3. This biomimetic system could be used for a quantitative evaluation of the early stage of virus infection or drug delivery.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.