ArticleJournal of virology2008
Monitoring early fusion dynamics of human immunodeficiency virus type 1 at single-molecule resolution.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- HIV-1 envelope glycoprotein modulates CXCR4 clustering and dynamics on the T cell membrane.eLife · 2026Article
- HIV-1 Entry and Membrane Fusion Inhibitors.Viruses · 2021Review
- Article
- Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding.Viruses · 2021Article
- The Interplay of HIV and Autophagy in Early Infection.Frontiers in microbiology · 2021Review
- Viral Membrane Fusion and the Transmembrane Domain.Viruses · 2020Review
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- Stoichiometric Analyses of Soluble CD4 to Native-like HIV-1 Envelope by Single-Molecule Fluorescence Spectroscopy.Cell reports · 2019Article
- Molecular Mechanism of HIV-1 Entry.Trends in microbiology · 2019Review
- Biomechanical characterization of TIM protein-mediated Ebola virus-host cell adhesion.Scientific reports · 2019Article
- A method for tethering single viral particles for virus-cell interaction studies with optical tweezers.Proceedings of SPIE--the International Society for Optical Engineering · 2018Article
- Combining confocal and atomic force microscopy to quantify single-virus binding to mammalian cell surfaces.Nature protocols · 2017Article
- Nanomechanical mapping of first binding steps of a virus to animal cells.Nature nanotechnology · 2017Article
- Review
- Bioinformatic analysis of HIV-1 entry and pathogenesis.Current HIV research · 2014Review
- β-Catenin serves as a clutch between low and high intercellular E-cadherin bond strengths.Biophysical journal · 2013Article
- Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins.Scientific reports · 2013Article
- Multiscale perspectives of virus entry via endocytosis.Virology journal · 2013Review
- Influenza virus binds its host cell using multiple dynamic interactions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular mechanistic insights into the endothelial receptor mediated cytoadherence of Plasmodium falciparum-infected erythrocytes.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The fusion of human immunodeficiency virus type 1 (HIV-1) to host cells is a dynamic process governed by the interaction between glycoproteins on the viral envelope and the major receptor, CD4, and coreceptor on the surface of the cell. How these receptors organize at the virion-cell interface to promote a fusion-competent site is not well understood. Using single-molecule force spectroscopy, we map the tensile strengths, lifetimes, and energy barriers of individual intermolecular bonds between CCR5-tropic HIV-1 gp120 and its receptors CD4 and CCR5 or CXCR4 as a function of the interaction time with the cell. According to the Bell model, at short times of contact between cell and virion, the gp120-CD4 bond is able to withstand forces up to 35 pN and has an initial lifetime of 0.27 s and an intermolecular length of interaction of 0.34 nm. The initial bond also has an energy barrier of 6.7 k(B)T (where k(B) is Boltzmann's constant and T is absolute temperature). However, within 0.3 s, individual gp120-CD4 bonds undergo rapid destabilization accompanied by a shortened lifetime and a lowered tensile strength. This destabilization is significantly enhanced by the coreceptor CCR5, not by CXCR4 or fusion inhibitors, which suggests that it is directly related to a conformational change in the gp120-CD4 bond. These measurements highlight the instability and low tensile strength of gp120-receptor bonds, uncover a synergistic role for CCR5 in the progression of the gp120-CD4 bond, and suggest that the cell-virus adhesion complex is functionally arranged about a long-lived gp120-coreceptor bond.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.