ArticleMolecular biology of the cell2008
Microtubule plus-end conformations and dynamics in the periphery of interphase mouse fibroblasts.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- On the curvature and relaxation of microtubule plus-end tips.Biophysical journal · 2026Article
- Theory of tip structure-dependent microtubule catastrophes and damage-induced microtubule rescues.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bending-torsional elasticity and energetics of the plus-end microtubule tip.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Microtubule assembly and disassembly dynamics model: Exploring dynamic instability and identifying features of Microtubules' Growth, Catastrophe, Shortening, and Rescue.Computational and structural biotechnology journal · 2022Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- Article
- Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography.Nature communications · 2020Article
- Mechanics and kinetics of dynamic instability.eLife · 2020Article
- Microtubule Plus End Dynamics - Do We Know How Microtubules Grow?: Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- Microtubules grow by the addition of bent guanosine triphosphate tubulin to the tips of curved protofilaments.The Journal of cell biology · 2018Article
- Microtubule aging probed by microfluidics-assisted tubulin washout.Molecular biology of the cell · 2016Article
- End-binding protein 1 stimulates paclitaxel sensitivity in breast cancer by promoting its actions toward microtubule assembly and stability.Protein & cell · 2014Article
- The mechanisms of microtubule catastrophe and rescue: implications from analysis of a dimer-scale computational model.Molecular biology of the cell · 2012Article
- Electron tomography reveals a flared morphology on growing microtubule ends.Journal of cell science · 2011Article
- Mechanochemical modeling of dynamic microtubule growth involving sheet-to-tube transition.PloS one · 2011Article
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Authors and funding
5 authors.
Funding
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Abstract
The plus ends of microtubules (MTs) alternate between phases of growth, pause, and shrinkage, a process called "dynamic instability." Cryo-EM of in vitro-assembled MTs indicates that the dynamic state of the plus end corresponds with a particular MT plus-end conformation. Frayed ("ram's horn like"), blunt, and sheet conformations are associated with shrinking, pausing, and elongating plus ends, respectively. A number of new conformations have recently been found in situ but their dynamic states remained to be confirmed. Here, we investigated the dynamics of MT plus ends in the peripheral area of interphase mouse fibroblasts (3T3s) using electron microscopical and tomographical analysis of cryo-fixed, freeze-substituted, and flat-embedded sections. We identified nine morphologically distinct plus-end conformations. The frequency of these conformations correlates with their proximity to the cell border, indicating that the dynamic status of a plus end is influenced by features present in the periphery. Shifting dynamic instability toward depolymerization with nocodazole enabled us to address the dynamic status of these conformations. We suggest a new transition path from growth to shrinkage via the so-called sheet-frayed and flared ends, and we present a kinetic model that describes the chronology of events taking place in nocodazole-induced MT depolymerization.
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