ArticlePlant physiology2008
An actin-binding protein, LlLIM1, mediates calcium and hydrogen regulation of actin dynamics in pollen tubes.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 112 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Genome Wide Identification and Characterization of LIM Domain Associated Gene Superfamily in Common Bean (International journal of genomics · 2025Article
- Autophagy modulates Arabidopsis male gametophyte fertility and controls actin organization.Nature communications · 2024Article
- Arabidopsis Actin-Binding Protein WLIM2A Links PAMP-Triggered Immunity and Cytoskeleton Organization.International journal of molecular sciences · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Expression of Clementine Asp-Rich Proteins (CcASP-RICH) in Tobacco Plants Interferes with the Mechanism of Pollen Tube Growth.International journal of molecular sciences · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.Horticulture research · 2021Article
- An optimized genetically encoded dual reporter for simultaneous ratio imaging of CaThe New phytologist · 2021Article
- Article
- Exocytosis and endocytosis: coordinating and fine-tuning the polar tip growth domain in pollen tubes.Journal of experimental botany · 2020Review
- Chloroplast avoidance movement: a novel paradigm of ROS signalling.Photosynthesis research · 2020Review
- Secretory Vesicles Targeted to Plasma Membrane During Pollen Germination and Tube Growth.Frontiers in cell and developmental biology · 2020Review
- Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes.Frontiers in cell and developmental biology · 2020Review
- Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor.Nanomedicine : nanotechnology, biology, and medicine · 2019Article
- The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.iScience · 2019Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2019Article
- Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting KPlant reproduction · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin microfilaments are crucial for polar cell tip growth, and their configurations and dynamics are regulated by the actions of various actin-binding proteins (ABPs). We explored the function of a lily (Lilium longiflorum) pollen-enriched LIM domain-containing protein, LlLIM1, in regulating the actin dynamics in elongating pollen tube. Cytological and biochemical assays verified LlLIM1 functioning as an ABP, promoting filamentous actin (F-actin) bundle assembly and protecting F-actin against latrunculin B-mediated depolymerization. Overexpressed LlLIM1 significantly disturbed pollen tube growth and morphology, with multiple tubes protruding from one pollen grain and coaggregation of FM4-64-labeled vesicles and Golgi apparatuses at the subapex of the tube tip. Moderate expression of LlLIM1 induced an oscillatory formation of asterisk-shaped F-actin aggregates that oscillated with growth period but in different phases at the subapical region. These results suggest that the formation of LlLIM1-mediated overstabilized F-actin bundles interfered with endomembrane trafficking to result in growth retardation. Cosedimentation assays revealed that the binding affinity of LlLIM1 to F-actin was simultaneously regulated by both pH and Ca(2+): LlLIM1 showed a preference for F-actin binding under low pH and low Ca(2+) concentration. The potential functions of LlLIM1 as an ABP sensitive to pH and calcium in integrating endomembrane trafficking, oscillatory pH, and calcium circumstances to regulate tip-focused pollen tube growth are discussed.
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