ArticleAmerican journal of physiology. Cell physiology2008
Effect of low pH on single skeletal muscle myosin mechanics and kinetics.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 3 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 3 syntheses or guidelines pooled it, 109 citations in OpenAlex.
- Pooled it
- Effects of Resistance Training to Muscle Failure on Acute Fatigue: A Systematic Review and Meta-Analysis.Sports medicine (Auckland, N.Z.) · 2022Pooled it
- Optimal Training Sequences to Develop Lower Body Force, Velocity, Power, and Jump Height: A Systematic Review with Meta-Analysis.Sports medicine (Auckland, N.Z.) · 2021Pooled it
- Beneficial effects of oral and topical sodium bicarbonate during a battery of team sport-specific exercise tests in recreationally trained male athletes.Journal of the International Society of Sports Nutrition · 2023Trial
- Beta-Alanine Supplementation and Sport Climbing Performance.International journal of environmental research and public health · 2021Trial
- Sodium citrate supplementation enhances tennis skill performance: a crossover, placebo-controlled, double blind study.Journal of the International Society of Sports Nutrition · 2019Trial
- The effect of pH on fatigue during submaximal isometric contractions of the human calf muscle.European journal of applied physiology · 2015Trial
- Common questions and misconceptions of sodium bicarbonate as an ergogenic aid: what does the science really show?Journal of the International Society of Sports Nutrition · 2026Review
- Phosphate and acidosis cause fibre type-specific changes to cellular and molecular contractile mechanics at 37°C in skeletal muscle from older adults.Experimental physiology · 2026Article
- A primer on the methods of skeletal and cardiac muscle mechanics using permeabilized preparations.The Journal of general physiology · 2026Review
- Profiling the epigenomic landscape of late embryonic and adult mouse hind limb muscles.Scientific reports · 2026Article
- Carnosine enhances actomyosin-ATPase activity under acidic conditions: the role of carnosine in a rigor mortis.Journal of animal science · 2026Article
- Carbon Dioxide Inhalation-Risks for Health or Opportunity for Physical Fitness Development?Journal of clinical medicine · 2026Review
- 24-hourFrontiers in transplantation · 2026Article
- Actomyosin-Based Nanodevices for Sensing and Actuation: Bridging Biology and Bioengineering.Biosensors · 2025Review
- Mechanisms for the age-related increase in fatigability of the elbow flexor muscles in healthy adults.Physiological reports · 2025Article
- A human skeletal muscle cross-bridge model to characterize the role of metabolite accumulation in muscle fatigue.Experimental physiology · 2025Article
- CaBiomolecules · 2025Review
- Lactic acidosis: implications for human exercise performance.European journal of applied physiology · 2025Review
- Impact of physiological ionic strength and crowding on kinesin-1 motility.Cell structure and function · 2025Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Acidosis (low pH) is the oldest putative agent of muscular fatigue, but the molecular mechanism underlying its depressive effect on muscular performance remains unresolved. Therefore, the effect of low pH on the molecular mechanics and kinetics of chicken skeletal muscle myosin was studied using in vitro motility (IVM) and single molecule laser trap assays. Decreasing pH from 7.4 to 6.4 at saturating ATP slowed actin filament velocity (V(actin)) in the IVM by 36%. Single molecule experiments, at 1 microM ATP, decreased the average unitary step size of myosin (d) from 10 +/- 2 nm (pH 7.4) to 2 +/- 1 nm (pH 6.4). Individual binding events at low pH were consistent with the presence of a population of both productive (average d = 10 nm) and nonproductive (average d = 0 nm) actomyosin interactions. Raising the ATP concentration from 1 microM to 1 mM at pH 6.4 restored d (9 +/- 3 nm), suggesting that the lifetime of the nonproductive interactions is solely dependent on the [ATP]. V(actin), however, was not restored by raising the [ATP] (1-10 mM) in the IVM assay, suggesting that low pH also prolongs actin strong binding (t(on)). Measurement of t(on) as a function of the [ATP] in the single molecule assay suggested that acidosis prolongs t(on) by slowing the rate of ADP release. Thus, in a detachment limited model of motility (i.e., V(actin) approximately d/t(on)), a slowed rate of ADP release and the presence of nonproductive actomyosin interactions could account for the acidosis-induced decrease in V(actin), suggesting a molecular explanation for this component of muscular fatigue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.