ArticleProceedings of the National Academy of Sciences of the United States of America2008
A gastrolith protein serving a dual role in the formation of an amorphous mineral containing extracellular matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 106 citations in OpenAlex.
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- Mineral formation in the primary polyps of pocilloporoid corals.Acta biomaterialia · 2019Article
- A diecast mineralization process forms the tough mantis shrimp dactyl club.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Identification and Characterization of theInternational journal of molecular sciences · 2019Article
- Minerals in the pre-settled coral Stylophora pistillata crystallize via protein and ion changes.Nature communications · 2018Article
- CPAP3 proteins in the mineralized cuticle of a decapod crustacean.Scientific reports · 2018Article
- Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei.Scientific reports · 2017Article
- Redefining metamorphosis in spiny lobsters: molecular analysis of the phyllosoma to puerulus transition in Sagmariasus verreauxi.Scientific reports · 2015Article
- Microarray: a global analysis of biomineralization-related gene expression profiles during larval development in the pearl oyster, Pinctada fucata.BMC genomics · 2015Article
- Dual Roles of the Lysine-Rich Matrix Protein (KRMP)-3 in Shell Formation of Pearl Oyster, Pinctada fucata.PloS one · 2015Article
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- Article
- Comparative ultrastructure and carbohydrate composition of gastroliths from astacidae, cambaridae and parastacidae freshwater crayfish (crustacea, decapoda).Biomolecules · 2012Article
- Enamel-like apatite crown covering amorphous mineral in a crayfish mandible.Nature communications · 2012Article
- Novel basic protein, PfN23, functions as key macromolecule during nacre formation.The Journal of biological chemistry · 2012Article
- Identification and characterization of differentially expressed transcripts in the gills of freshwater prawn (Macrobrachium rosenbergii) under salt stress.TheScientificWorldJournal · 2012Article
- Ubiquitylation functions in the calcium carbonate biomineralization in the extracellular matrix.PloS one · 2012Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the proclamation of Lowenstam and Weiner that crustaceans are the "champions of mineral mobilization and deposition of the animal kingdom," relatively few proteins from the two main calcification sites in these animals, i.e., the exoskeleton and the transient calcium storage organs, have been identified, sequenced, and their roles elucidated. Here, a 65-kDa protein (GAP 65) from the gastrolith of the crayfish, Cherax quadricarinatus, is fully characterized and its function in the mineralization of amorphous calcium carbonate (ACC) of the extracellular matrix is demonstrated. GAP 65 is a negatively charged glycoprotein that possesses three predicted domains: a chitin-binding domain 2, a low-density lipoprotein receptor class A domain, and a polysaccharide deacetylase domain. Expression of GAP 65 was localized to columnar epithelial cells of the gastrolith disk during premolt. In vivo administration of GAP 65 dsRNA resulted in a significant reduction of GAP 65 transcript levels in the gastrolith disk. Such gene silencing also caused dramatic structural and morphological deformities in the chitinous-ACC extracellular matrix structure. ACC deposited in these gastroliths appeared to be sparsely packed with large elongated cavities compared with the normal gastrolith, where ACC is densely compacted. ACC spherules deposited in these gastroliths are significantly larger than normal. GAP 65, moreover, inhibited calcium carbonate crystallization in vitro and stabilized synthetic ACC. Thus, GAP 65 is the first protein shown to have dual function, involved both in extracellular matrix formation and in mineral deposition during biomineralization.
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