ArticleClinical and vaccine immunology : CVI2008
Genetic and antigenic diversities of major immunoreactive proteins in globally distributed Ehrlichia canis strains.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Review
- TRP36-ELISA forHeliyon · 2024Article
- Immuno- and expression analysis ofFrontiers in veterinary science · 2024Article
- Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand.Scientific reports · 2023Article
- Costa Rican Genotype ofVeterinary sciences · 2023Article
- Antibody reactive immunomes ofFrontiers in cellular and infection microbiology · 2023Article
- Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba.Parasites & vectors · 2022Article
- RecombinantVeterinary sciences · 2022Article
- Exposure of Domestic Cats to DistinctVeterinary sciences · 2021Article
- Effect of GP19 Peptide Hyperimmune Antiserum on Activated Macrophage duringAnimals : an open access journal from MDPI · 2021Article
- Geographic Distribution ofVeterinary sciences · 2020Article
- Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the Citrate Synthase Gene for Detection ofVeterinary sciences · 2020Article
- Molecular Detection and Characterization of theFrontiers in veterinary science · 2020Article
- Ehrlichia chaffeensis Outer Membrane Protein 1-Specific Human Antibody-Mediated Immunity Is Defined by Intracellular TRIM21-Dependent Innate Immune Activation and Extracellular Neutralization.Infection and immunity · 2019Article
- Low genetic diversity of Ehrlichia canis associated with high co-infection rates in Rhipicephalus sanguineus (s.l.).Parasites & vectors · 2019Article
- Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Mini-review: Strategies for Variation and Evolution of Bacterial Antigens.Computational and structural biotechnology journal · 2015Review
- Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil.Journal of veterinary science · 2014Article
- New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats.Parasites & vectors · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
The extent of knowledge regarding the diversity of globally distributed Ehrlichia canis strains has been limited to information gained from a few evolutionarily conserved genes. In this study, E. canis strains from the United States (strain Jake [US]), Brazil (strain São Paulo [BR]), and Israel (strain 611 [IS] and Ranana [IS-R]) were used to examine the antigenic and genetic diversities of four well-characterized major immunoreactive protein genes/proteins. gp36 and gp200 were the most divergent genes, and nucleotide substitutions in the gp36 tandem repeat region of the IS strain, but not the IS-R strain, resulted in two amino acid differences (S-->P and P-->T) in each nine-amino-acid repeat (epitope-containing region). DNA sequences of gp19 and gp140 were completely conserved in the US and BR strains, but differences were found in the Israeli strains, including two fewer tandem repeats in gp140 and a single amino acid substitution in gp19 from the IS strain. E. canis whole-cell lysates from each isolate were examined by Western immunoblotting using sera from naturally infected dogs from each country, and four major immunoreactive proteins (gp19, gp36, gp140, and gp200) were identified in each strain using protein-specific antisera. The US and BR strains exhibited highly conserved immunoreactive protein profiles, while some differences were identified in the IS strain. Sera from naturally infected Israeli dogs confirmed gene sequencing information, which demonstrated two distinct E. canis strains, defined by the gp36 gene. Conversely, gp19 was strongly reactive and present in all E. canis isolates. gp140 and gp200 were also present in all strains, although gp140 in the IS strain had two fewer tandem repeats and exhibited a smaller mass.
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