ArticleNucleic acids research2008
A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Methylation of the Glucocorticoid Receptor Gene in Children with Somatic Symptom Disorder: A Case-Control Study.Epigenomes · 2025Article
- Article
- DNA Demethylation Switches Oncogenic ΔNp63 to Tumor Suppressive TAp63 in Squamous Cell Carcinoma.Frontiers in oncology · 2022Article
- DNA hypermethylation/boundary control loss identified in retinoblastomas associated with genetic and epigenetic inactivation of theEpigenetics · 2021Article
- Double signal enhancement strategy based on rolling circle amplification and photoinduced electron transfer for ultrasensitive fluorometric detection of methylated DNA.Mikrochimica acta · 2018Article
- Age-related methylation profiles of equine blood leukocytes in the RNASEL locus.Journal of applied genetics · 2016Article
- The Role of Embryonic Stem Cell-expressed RAS (ERAS) in the Maintenance of Quiescent Hepatic Stellate Cells.The Journal of biological chemistry · 2016Article
- Epigenetic structure and the role of polymorphism in the shaping of DNA methylation patterns of equine OAS1 locus.Journal of applied genetics · 2015Article
- CHST11 gene expression and DNA methylation in breast cancer.International journal of oncology · 2015Article
- Epigenetic Changes during Hepatic Stellate Cell Activation.PloS one · 2015Article
- Postnatal epigenetic modification of glucocorticoid receptor gene in preterm infants: a prospective cohort study.BMJ open · 2014Article
- Evidence for regulation of UDP-glucuronosyltransferase (UGT) 1A1 protein expression and activity via DNA methylation in healthy human livers.The Journal of pharmacy and pharmacology · 2013Article
- Epigenetic alterations may regulate temporary reversal of CD4(+) T cell activation caused by trichloroethylene exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Subtle decreases in DNA methylation and gene expression at the mouse Igf2 locus following prenatal alcohol exposure: effects of a methyl-supplemented diet.Alcohol (Fayetteville, N.Y.) · 2011Article
- In vivo methylation of mtDNA reveals the dynamics of protein-mtDNA interactions.Nucleic acids research · 2009Article
- A method to quantify mouse coat-color proportions.PloS one · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
DNA methylation at cytosines is a widely studied epigenetic modification. Methylation is commonly detected using bisulfite modification of DNA followed by PCR and additional techniques such as restriction digestion or sequencing. These additional techniques are either laborious, require specialized equipment, or are not quantitative. Here we describe a simple algorithm that yields quantitative results from analysis of conventional four-dye-trace sequencing. We call this method Mquant and we compare it with the established laboratory method of combined bisulfite restriction assay (COBRA). This analysis of sequencing electropherograms provides a simple, easily applied method to quantify DNA methylation at specific CpG sites.
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